miRNA Biogenesis : Methods and Protocols - Ørom, Ulf Andersson Vang.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Springer, 2018)
High-throughput characterization of primary microRNA transcripts -- Identifying pri-miRNA transcription start sites -- Metabolic pulse-chase RNA labelling for pri-miRNA processing dynamics -- In Vitro System for Coupling RNAP II Transcription to Primary microRNA Processing and a Three-way System for RNAP II Transcription/Splicing/microRNA Processing -- Purification of Microprocessor-associated factors -- Arresting pri-miRNA processing with Target Site Blockers -- MicroRNA analysis using the quantitative real-time PCR reaction -- MicroRNA analysis using next-generation sequencing -- Identification of microRNA precursor-associated proteins -- Analysis of 3' end modifications in (precursor-) microRNAs by high-throughput -- Uncovering the stability of mature miRNAs by 4-thio-uridine metabolic labeling -- Detection of microRNA-target interactions by Chimera PCR (ChimP) -- High-quality overlapping paired-end reads for the detection of A-to-I editing on small RNA -- Targeting miRNA for therapeutics using a Micronome based method for identification of miRNA-mRNA pairs and validation of key regulator miRNA -- Method for detection of miRNAs in non-model organisms with unreported database -- Detection and Verification of Mammalian Mirtrons by Northern Blotting -- Detecting agotrons in Ago CLIPseq data.
Keywords: Human genetics; Human Genetics