Western Blotting : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2015.
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| Edición: | 1st ed. 2015. |
| Colección: | Methods in Molecular Biology,
1312 |
| Materias: |
Tabla de Contenidos:
- The Early Days of Blotting
- Origins of Protein Blotting
- Western Blotting: Remembrance of Things Past
- Simian Virus 40 and Protein Transfer
- Western Blotting: An Introduction
- From Little Helpers to Automation
- Spectrophotometric Methods to Determine Protein Concentration
- Solubilization of Proteins: The Importance of Lysis Buffer Choice
- Simultaneous Immunoblotting Analysis with Activity Gel Electrophoresis and 2-D Gel Electrophoresis
- Diffusion Blotting: A Rapid and Simple Method for Production of Multiple Blots from a Single Gel
- Multiple Immunoblots by Passive Diffusion of Proteins from a Single SDS-PAGE Gel
- Slice Blotting
- Localizing Proteins by Tissue Printing
- Dot-Immunobinding Assay (Dot-Iba)
- Analysis of Antibody Clonotype by Affinity Immunoblotting
- Glycosaminoglycan Blotting and Detection after Electrophoresis Separation
- A Well-Based Reverse-Phase Protein Array of Formalin-Fixed Paraffin-Embedded Tissue
- Quantitative Computerized Western Blotting in Detail
- Cationic Electrophoresis and Eastern Blotting
- A Miniaturized Blotting System for Simultaneous Detecting of Different Autoantibodies
- Proteomic Expressional Profiling of a Paraffin Embedded Tissue by Multiplex Tissue Immunoblotting
- Post-Staining Electroblotting for Efficient and Reliable Peptide Blotting
- Multistrip Western Blotting: A Tool for Comparative Quantitative Analysis of Multiple Proteins
- Western Blotting using PVDF Membranes and Its Downstream Applications
- Blotting from PhastGel to Membranes by Ultrasound
- Western Blotting of High and Low Molecular Weight Proteins Using Heat
- Membrane Strip Affinity Purification of Autoantibodies
- Strip Immunoblotting of Multiple Antigenic Peptides
- Double-Blotting: A Solution to the Problem of Non-Specific Binding of Secondary Antibodies in Immunoblotting Procedures
- Method for Resolution and Western Blotting of Very Large Proteins Using Agarose Electrophoresis
- Immunodetection of P-Selectin Using an Antibody to its C-Terminal Tag
- Improvements and Variants of the Mutliple Antigen Blot Assay-MABA: An Immunoenzymatic Technique for Simultaneous Antigen and Antibody Screening
- Blotting from Immobilized pH Gradient Gels: Application to Total Cell Lysates
- Immunoprecipitation: Western Blot for Proteins of Low Abundance
- Native Electrophoresis and Western Blot Analysis (NEWeB): Methods and Applications
- Shift-Western Blotting: Separate Analysis of Protein and DNA from Protein-DNA Complexes
- Grid-Immunoblotting
- Detection and Quantification of Protein-Protein Interactions by Far-Western Blotting
- Western Blot Analysis of Adhesive Interactions under Fluid Shear Conditions: The Blot Rolling Assay
- Centrifuge Blotting
- Blotting of Coomassie Blue Stained Proteins from PAGE Gels to Transparencies
- B Cell ELISPOT: For the Identification of Antigen-Specific Antibody Secreting Cells
- T Cell ELISPOT: For the Identification of Specific Cytokine-Secreting T Cells
- Membrane Microplates for One- and Two-Color ELISPOT and FLUOROSPOT Assays
- SDS PAGE to Immunoblot in One Hour
- Single Cell Western Blotting
- Protein Detection by Simple Western™ Analysis
- Western Blotting Using Microfluidics
- Two-Dimensional Gel-Based Protein Standardization Verified by Western Blot Analysis
- Fingerprint Deposition of Nitrocellulose and Polyvinylidene Difluoride Membranes Using Alkaline Phosphatase
- Other Notable Protein Blotting Methods: A Brief Review. .