Yeast Protocols - Xiao, Wei.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2014)
Basic Investigations in Saccharomyces cerevisiae -- Isolation of Yeast Nucleic Acids -- Quantification of GFP Signals by Fluorescent Microscopy and Flow Cytometry -- Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method -- Targeted Gene Deletion in Saccharomyces cerevisiae and Schizosaccharomyces pombe -- See & Catch Method for Studying Protein Complexes in Yeast Cells: A Technique Unifying Fluorescence Microscopy and Mass Spectrometry -- Yeast Two Hybrid Liquid Screening -- Targeted Mutagenesis of a Specific Gene in Yeast -- Fluorescence Staining of Mitochondria for Morphology Analysis in S. cerevisiae -- Assays for Autophagy I: the Cvt Pathway and Non-Selective Autophagy -- Assays for Autophagy II: Mitochondrial Autophagy -- Biochemically Characterizing the Subcellular Localization of Peroxisomal Proteins by Fractionation, Protease Protection and Carbonate Extraction -- Yeast Survival and Growth Assays -- Spontaneous Mutagenesis Assay -- Mutational Specificity Analysis: Assay for Mutations in the Yeast SUP4-o Gene -- piggyBac Transposon-Based Insertional Mutagenesis for the Fission Yeast Schizosaccharomyces pombe -- Replicative and Chronological Life Span Assays -- Detection of Protein Arginine Methylation in Saccharomyces cerevisiae -- Detection of Protein Post-translational Modifications from Whole Cell Extracts in Saccharomyces cerevisiae -- Ligand Engineering Using Yeast Surface Display -- Screening Combinatorial Libraries of Cyclic Peptides Using the Yeast Two-Hybrid Assay.
Keywords: Microbiology; Microbiology