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171213s2018 xxu| s |||| 0|eng d |
| 020 |
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|a 9781493975402
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| 024 |
7 |
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|a 10.1007/978-1-4939-7540-2
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a mRNA Decay :
|b Methods and Protocols /
|c edited by Shireen R. Lamandé.
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| 250 |
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|a 1st ed. 2018.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2018.
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| 300 |
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|a XI, 227 p. 34 illus., 24 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1720
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| 505 |
0 |
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|a 5'bromouridine IP Chase (BRIC)-Seq to Determine RNA Half Lives -- Determining mRNA Decay Rates Using RNA Approach to Equilibrium Sequencing (RATE-Seq) -- Metabolic Labeling of Newly Synthesized RNA with 4sU to in Parallel Assess RNA Transcription and Decay -- Measuring mRNA Decay in Budding Yeast Using Single Molecule FISH -- PAR-CLIP for Discovering Target Sites of RNA-Binding Proteins -- Characterizing mRNA Sequence Motifs in the 3'UTR Using GFP Reporter Constructs -- iCLIP of the PIWI Protein Aubergine in Drosophila Embryos -- Integration of ENCODE RNAseq and eCLIP Data Sets -- Identifying miRNA Targets Using AgoRIP-Seq.- Integrated Analysis of miRNA and mRNA Expression Profiles to Identify miRNA Targets -- Identifying RISC Components Using Ago2 Immunoprecipitation and Mass Spectrometry -- Using Tet-Off Cells and RNAi Knockdown to Assay mRNA Decay -- Identifying Cellular Nonsense-Mediated mRNA Decay (NMD) Targets: Immunoprecipitation of Phosphorylated UPF1 Followed by RNA Sequencing (p-UPF1 RIP-Seq) -- Generation of Cell Lines Stably Expressing a Fluorescent Reporter of Nonsense-Mediated mRNA Decay Activity -- Reactivation Assay to Identify Direct Targets of the Nonsense-Mediated mRNA Decay Pathway in Drosophila.- Studying Nonsense-Mediated mRNA Decay in Mammalian Cells Using a Multicolored Bioluminescence-Based Reporter System.
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| 650 |
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|a Human genetics.
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| 650 |
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4 |
|a Human Genetics.
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| 700 |
1 |
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|a Lamandé, Shireen R.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
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