mRNA Decay : Methods and Protocols - Lamandé, Shireen R.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2018)
5'bromouridine IP Chase (BRIC)-Seq to Determine RNA Half Lives -- Determining mRNA Decay Rates Using RNA Approach to Equilibrium Sequencing (RATE-Seq) -- Metabolic Labeling of Newly Synthesized RNA with 4sU to in Parallel Assess RNA Transcription and Decay -- Measuring mRNA Decay in Budding Yeast Using Single Molecule FISH -- PAR-CLIP for Discovering Target Sites of RNA-Binding Proteins -- Characterizing mRNA Sequence Motifs in the 3'UTR Using GFP Reporter Constructs -- iCLIP of the PIWI Protein Aubergine in Drosophila Embryos -- Integration of ENCODE RNAseq and eCLIP Data Sets -- Identifying miRNA Targets Using AgoRIP-Seq.- Integrated Analysis of miRNA and mRNA Expression Profiles to Identify miRNA Targets -- Identifying RISC Components Using Ago2 Immunoprecipitation and Mass Spectrometry -- Using Tet-Off Cells and RNAi Knockdown to Assay mRNA Decay -- Identifying Cellular Nonsense-Mediated mRNA Decay (NMD) Targets: Immunoprecipitation of Phosphorylated UPF1 Followed by RNA Sequencing (p-UPF1 RIP-Seq) -- Generation of Cell Lines Stably Expressing a Fluorescent Reporter of Nonsense-Mediated mRNA Decay Activity -- Reactivation Assay to Identify Direct Targets of the Nonsense-Mediated mRNA Decay Pathway in Drosophila.- Studying Nonsense-Mediated mRNA Decay in Mammalian Cells Using a Multicolored Bioluminescence-Based Reporter System.
Keywords: Human genetics; Human Genetics