Reviews and Protocols in DT40 Research Subcellular Biochemistry - Buerstedde, Jean-Marie.; Takeda, Shunichi; SpringerLink (Online service) (Dordrecht : Springer Netherlands : Imprint: Springer, 2006)
DT40 gene disruptions: a how-to for the design and the construction of targeting vectors -- Immunoglobulin gene conversion or hypermutation: that's the question -- Genome resources for the DT40 community -- Chromosome engineering in DT40 cells and mammalian centromere function -- Function of RECQ family helicase in genome stability -- Genetic analysis of apoptotic execution -- The DT40 system as a tool for analyzing kinetochore assembly -- Analysing the DNA damage and replication checkpoints in DT40 cells -- Using DT40 to study clathrin function -- Genetic analysis of B cell signaling -- DT40 mutants: a model to study transcriptional regulation of B cell development and function -- Transcription and RNA processing factors play complex roles in DT40 cells -- Participation of histones, histone modifying enzymes and histone chaperonesin vertebrate cell functions -- Analysis of gene expression, copy number and palindrome formation with a DT40 enriched CDNA microarray -- Calcium signaling, ion channels and more -- Analysis of DNA replication damage bypass and its role in immunoglobulin repertoire development -- The fanconi anemia pathway promotes homologous recombination repair in DT40 cell line -- Phenotypic analysis of cellular responses to DNA damage -- ATM, a paradigm for a stress-responsive signal transducer in higher vertebrate cells -- Stable non-targeted transfection of DT40 -- Basic cell culture conditions -- Excision of floxed-DNA sequences by transient induction of MER-CRE-MER -- Immunoglobulin gene conversion and hypermutation assay by facs -- Target screening by PCR -- Mitotic index determination by flow cytometry -- Centrifugal elutriation as a means of cell cycle phase separation and synchronisation -- Preparation of genomic DNA for microarray-based comparative genome hybridization -- Analysis of cellular Mg2+ in DT40 cells -- Transient transfection of DT40 -- Retroviral transduction of DT40 -- Colony survival assay -- Subcloning DT40 by limiting dilution -- Subnuclear immunofluorescence -- Sister chromatid exchange assay -- 2D cell cycle analysis -- Purification of tap-tagged proteins by two-step pull down from DT40 cells -- Synchronization of cells -- Targeted transfection of DT40 cells -- Luciferase reporter assay -- Indirect immunofluorescence microscopy -- Quantification of receptor-mediated endocytosis -- Measurement of DNA synthesis and strand breaks using alkaline sucrose density gradient centrifugation -- Isolation of nuclear and cytoplasmic proteins from DT40 cell lines.
Keywords: Cell biology; Cancer research; Biochemistry; Life sciences; Vertebrates; Cell Biology; Cancer Research; Biochemistry, general; Life Sciences, general; Vertebrates