Principles and Technical Aspects of PCR Amplification - van Pelt-Verkuil, Elizabeth; Belkum, Alex van.; Hays, John P.; SpringerLink (Online service) (Dordrecht : Springer Netherlands : Imprint: Springer, 2008)
The Polymerase Chain Reaction -- A Brief Comparison Between In Vivo DNA Replication and In Vitro PCR Amplification -- The PCR in Practice -- The Different Types and Varieties of Nucleic Acid Target Molecules -- PCR Primers -- Deoxynucleotide Triphosphates and Buffer Components -- Taq and Other Thermostable DNA Polymerases -- Important Considerations for Typical, Quantitative and Real-Time PCR Protocols -- Analysis of PCR Amplification Products -- Ensuring PCR Quality - Laboratory Organisation, PCR Optimization and Controls -- Ensuring PCR Quality - Quality Criteria and Quality Assurance -- Variants and Adaptations of the Standard PCR Protocol -- In Situ PCR Amplification (ISA) - Major Considerations, Sample Processing and Applications.
Keywords: Life sciences; Medicine; Biochemistry; Cell biology; Biology-Technique.; Life Sciences, general; Biomedicine, general; Biochemistry, general; Cell Biology; Biological Techniques