Coronaviruses Methods and Protocols - Maier, Helena Jane.; Bickerton, Erica; Britton, Paul.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2015)
Coronaviruses: An Overview of Their Replication and Pathogenesis -- Identification of a Novel Coronavirus from Guinea Fowl Using Metagenomics -- Serological Diagnosis of Feline Coronavirus Infection by Immunochromatographic Test -- Estimation of Evolutionary Dynamics and Selection Pressure in Coronaviruses -- The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration -- The Preparation of Chicken Kidney Cell Cultures for Virus Propagation -- Isolation and Propagation of Coronaviruses in Embryonated Eggs -- Characterization of Human Coronaviruses on Well-Differentiated Human Airway Epithelial Cell Cultures -- Quantification of Infectious Bronchitis Coronaviruses by Titration in vitro and in ovo -- Purification of Coronavirus Virions for Cryo-EM and Proteomic Analysis -- Partial Purification of IBV and Subsequent Isolation of Viral RNA for Next Generation Sequencing -- Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses -- Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes -- Protein Histochemistry Using Coronaviral Spike Proteins: Studying Binding Profiles and Sialic Acid Requirements for Attachment to Tissues -- Identification of Protein Receptors for Coronaviruses by Mass Spectrometry -- Single Particle Tracking Assay to Study Coronavirus Membrane Fusion -- Studying Coronavirus-Host Protein Interactions -- A Field-Proven Yeast Two-Hybrid Protocol Used to Identify Coronavirus-Host Protein-Protein Interactions -- Investigation of the Functional Roles of Host Cell Proteins Involved in Coronavirus Infection Using Highly Specific and Scalable RNA Interference (RNAi) Approach -- Transcriptome Analysis of Feline Infectious Peritonitis Virus (FIPV) Infection -- Quantification of Interferon Signaling in Avian Cells -- Studying the Dynamics of Coronavirus Replicative Structures -- Preparation of Cultured Cells Using High Pressure Freezing and Freeze Substitution for Subsequent 2D or 3D Visualization in the Transmission Electron Microscope.
Keywords: Microbiology; Virology; Microbiology; Virology