Estrogen Receptors Methods and Protocols - Eyster, Kathleen M.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2016)
The Estrogen Receptors: An Overview from Different Perspectives -- Competitive Binding Assay for the G-Protein Coupled Receptor 30 (GPR30) or G-Protein Coupled Estrogen Receptor (GPER) -- The Use of Real Time Reverse Transcription-PCR for Assessing Estrogen Receptor and Estrogen-Responsive Gene Expression -- Bioinformatics Analysis of Estrogen-Responsive Genes -- Electrophoretic Mobility Shift Assay (EMSA) and Supershift Assay of Cytochrome P450 2B6 in Response to Estrogen -- Chromatin Immunoprecipitation Assay to Identify Genomic Binding Sites of Regulatory Factors -- Chromatin Immunoprecipitation with Estrogen Receptor 1 and the Promoter of Greb1 in TM4 Sertoli Cells -- Chromatin Immunoprecipitation-Sequencing (ChIP-seq) for Mapping of Estrogen Receptor-Chromatin Interactions in Breast Cancer -- RNA-Seq Experiment and Data Analysis -- DNA Microarray Analysis of Estrogen-Responsive Genes -- Shotgun Proteomics Analysis of Estrogen Effects in the Uterus Using Two Dimensional Liquid Chromatography and Tandem Mass Spectrometry -- Assessment of Protein Expression by Proximity Ligation Assay in the Nonhuman Primate Endometrium, Placenta, and Fetal Adrenal in Response to Estrogen -- Co-Localization of Estrogen Receptors with the Fluorescent Tamoxifen Derivative, FLTX1, Analyzed by Confocal Microscopy -- Live Cell Imaging of the Estrogen Receptor by Total Internal Reflection Fluorescence Microscopy -- In Situ Hybridization of Estrogen Receptors α and β and GPER in the Human Testis -- Purification of Histone Lysine Methyltransferase SMYD2 and Co-Crystallization with a Target Peptide from Estrogen Receptor a -- Gold Nanoparticle-Based Förster Resonance Energy Transfer (FRET) Analysis of Estrogen Receptor: DNA Interaction -- Analysis of Interaction of Estradiol with Estrogen Receptor by NMR Spectroscopy -- Application of Circular Dichroism Spectroscopy to the Analysis of the Interaction between the Estrogen Receptor Alpha and Co-Activators: The Case of Calmodulin -- Surface Plasmon Resonance Study of Cooperative Interactions of Estrogen Receptor α and Specificity Protein 1 with Composite DNA Elements -- Tips and Tricks for Successful Application of Statistical Methods to Biological Data -- The Synonymous Ala87 Mutation of Estrogen Receptor Alpha Modifies Transcriptional Activation through Both ERE and AP1 Sites -- Selective Estrogen Receptor Modulators and the Tissue-Selective Estrogen Complex: Analysis of Cell Type-Specific Effects using In Vivo Imaging of a Reporter Mouse Model -- Use of Reporter Genes to Analyze Estrogen Response: The Transgenic Zebrafish Model -- Comparison of the Effects of the Selective Estrogen Receptor Modulators Ospemifene, Raloxifene, and Tamoxifen on Breast Tissue in Ex Vivo Culture -- Estrogen Receptor Agonists and Antagonists in the Yeast Estrogen Bioassay -- Silencing Estrogen Receptor-α with siRNA in the Intact Rodent Brain -- Silencing Estrogen Receptor-β with siRNA in Cultured Cells -- Experimental Procedures for Demonstration of MicroRNA Mediated Enhancement of Functional Neuroprotective Effects of Estrogen Receptor Agonists -- Expression Profiles of Estrogen-Regulated MicroRNAs in Breast Cancer Cells -- Estradiol-Induced Transcriptional Regulation of Long Non-Coding RNA, HOTAIR.-Detection and Functional Analysis of Estrogen Receptor α Phosphorylated at Serine 216 in Mouse Neutrophils -- Estrogen Receptor-α Knockout Mice -- Detection of Endogenous Selective Estrogen Receptor Modulators such as 27-Hydroxycholesterol -- Phytoestrogens Activate the Estrogen Receptor in HepG2 Cells -- Detection of the Phosphorylation of the Estrogen Receptor a as an Outcome of GPR30 Activation -- GPER Mediates Non-Genomic Effects of Estrogen -- GPER/GPR30 Knockout Mice: Effects of GPER on Metabolism -- Analysis of G-Protein Coupled Receptor 30 (GPR30) on Endothelial Inflammation -- Atherosclerosis and Vascular Biologic Responses to Estrogens: Histologic, Immunohistochemical, Biochemical, and Molecular Methods -- Assessing Direct Vascular Actions of Estrogens -- Molecular Design, Synthesis, and Evaluation of SNIPER(ER) that Induces Proteasomal Degradation of ERα -- Regulation of Activation Induced Deaminase (AID) by Estrogen.
Keywords: Biochemistry; Endocrinology .; Biochemistry, general; Endocrinology