Lipid Signaling Protocols - Waugh, Mark.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2016)
Method for Assaying the Lipid Kinase Phosphatidylinositol-5-phosphate 4-kinase α in Quantitative High-Throughput Screening (qHTS) Bioluminescent Format -- Assaying Ceramide Synthase Activity In Vitro and in Living Cells using Liquid Chromatography-Mass Spectrometry -- Fluorescent Assays for Ceramide Synthase Activity -- Identification of the Interactome of a Palmitoylated Membrane Protein, Phosphatidylinositol 4-kinase type II Alpha -- Measurement of Long Chain Fatty Acyl-CoA synthetase Activity -- Qualitative and Quantitative in vitro Analysis of Phosphatidylinositol Phosphatase Substrate Specificity -- Luciferase Reporter Assays to Assess Liver X Receptor Transcriptional Activity -- Metabolically Biotinylated Reporters for Electron Microscopic Imaging of Cytoplasmic Membrane Microdomains -- Fluorescence Recovery after Photobleaching Analysis of the Diffusional Mobility of Plasma Membrane Proteins: HER3 Mobility in Breast Cancer Cell Membranes -- Isolation and Analysis of Detergent-Resistant Membrane Fractions -- Detection of Isolated Mitochondria-Associated ER Membranes using the Sigma-1 Receptor -- Using Surface Plasmon Resonance to Quantitatively Assess Lipid-Protein Interactions -- Analyzing Protein-phosphoinositide Interactions with Liposome Flotation Assays -- High-Throughput Flurometric Assay for Membrane-Protein Interaction -- Guidelines for the use of Protein Domains in Acidic Phospholipid Imaging -- Analysis of Sphingolipid Synthesis and Transport by Metabolic Labeling of Cultured Cells with [3H]serine -- Determination and Characterization of Tetraspanin-Associated Phosphoinositide-4 Kinases in Primary and Neoplastic Liver Cells -- Analysis of the Phosphoinositide Composition of Subcellular Membrane Fractions -- Single-molecule Imaging of Signal Transduction via GPI-anchored Receptors -- Measuring Phosphatidylinositol Generation on Biological Membranes -- Assay for CDP-diacylglycerol Generation by CDS in Membrane Fractions.
Keywords: Cell biology; Cell membranes .; Lipids; Cell Biology; Membrane Biology; Lipidology