Molecular Genetics of Asthma - Isidoro García, María.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2016)
Applications of Molecular Genetics to the Study of Asthma -- Assessment and Validation of New Genetic Variants: A systematic In Silico Approach -- Introduction to the Gene Expression Analysis -- Real Time PCR for Gene Expression Quantification in Asthma -- Validation of Reference Genes in mRNA Expression Analysis Applied to the Study of Asthma -- Review of Methods to Study Gene Expression Regulation Applied to Asthma -- Interactions of DNA and Proteins: Electrophoretic Mobility Shift Assay in Asthma -- SDS-Polyacrylamide Electrophoresis and Western Blotting Applied to the Study of Asthma -- Chromatin Immunoprecipitation: Application to the Study of Asthma -- Gene Silencing Delivery Methods: Lipid-Mediated and Electroporation Transfection Protocols -- Protocols for Exosome Isolation and RNA Profiling -- Cell Culture Techniques: Corticosteroid Treatment in A549 Human Lung Epithelial Cell -- Protocol for Lipid-Mediated Transient Transfection in A549 Epithelial Lung Cell Line -- Promoter Assay Using Luciferase Reporter Gene in the A549 Cell Line -- Review of Mouse Models Applied to the Study of Asthma -- General and Specific Mouse Models for Asthma Research -- Mouse Models applied to the Research of Pharmacological Treatments in Asthma -- Review on Pharmacogenetics and Pharmacogenomics Applied to the Study of Asthma.
Keywords: Molecular biology; Molecular Medicine