Histone Deacetylases Methods and Protocols - Sarkar, Sibaji; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2016)
A Sensitive and Flexible Assay for Determining Histone Deacetylase 1 (HDAC1) Activity -- Detection of Sumo Modification of Endogenous Histone Deacetylase 2 (HDAC2) in Mammalian Cells -- Analysis of Epigenetic Regulation of Hypoxia-Induced Epithelial-Mesenchymal Transition in Cancer Cells by Quantitative Chromatin Immunoprecipitation of Histone Deacetylase 3 (HDAC3) -- Molecular and Functional Characterization of Histone Deacetylase 4 (HDAC4) -- Approaches for Studying the Subcellular Localization, Interactions, and Regulation of Histone Deacetylase 5 (HDAC5) -- Analysis of Expression and Functions of Histone Deacetylase 6 (HDAC 6) -- Analysis of Histone Deacetylase 7 (HDAC7) Alternative Splicing and Its Role in Embryonic Stem Cell Differentiation toward Smooth Muscle Lineage -- Large-Scale Overproduction and Purification of Recombinant Histone Deacetylase 8 (HDAC8) from the Human-Pathogenic Flatworm Schistosoma mansoni -- Visualization of Histone Deacetylase 9 (HDAC9) Spatiotemporal Subcellular Localization in Primary Neuron Cultures -- Expression and Function of Histone Deacetylase 10 (HDAC10) in B Cell Malignancies -- Functional Analysis of Histone Deacetylase 11 (HDAC11) -- Sirtuin1 (SIRT1) in the Acetylation of Downstream Target Proteins -- Protocols for Cloning, Expression, and Functional Analysis of Sirtuin2 (SIRT2) -- Cloning and Characterization of Sirtuin3 (SIRT3) -- Identification of Sirtuin4 (SIRT4) Protein Interactions: Uncovering Candidate Acyl-Modified Mitochondrial Substrates and Enzymatic Regulators -- Generation and Purification of Catalytically Active Recombinant Sirtuin5 (SIRT5) Protein -- Sirtuin6 (SIRT6) Activity Assays -- Molecular, Cellular, and Physiological Characterization of Sirtuin7 (SIRT7) -- HDAC Inhibitors -- Assessment of the Anti-Proliferative Activity of a BET Bromodomain Inhibitor as Single Agent and in Combination in Non-Hodgkin Lymphoma Cell Lines -- Screening the Impact of Sirtuin Inhibitors on Inflammatory and Innate Immune Responses of Macrophages and in a Mouse Model of Endotoxic Shock. .
Keywords: Cell biology; Cell Biology