Natural Killer Cells Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Somanchi, Srinivas S. (Editor)
Format: eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Humana, 2016.
Edition:1st ed. 2016.
Series:Methods in Molecular Biology, 1441
Subjects:
Online Access:https://doi.org/10.1007/978-1-4939-3684-7
Table of Contents:
  • Tracking Effector and Memory NK Cells During MCMV Infection
  • Application of Mass Cytometry (CyTOF) for Functional and Phenotypic Analysis of Natural Killer Cells
  • Assessment of NK Cell Metabolism
  • Genotyping Single Nucleotide Polymorphisms and Copy Number Variability of the FCGRs Expressed on NK Cells
  • Measurement of Average Telomere Length in Ex Vivo Expanded Natural Killer Cells by Fluorescence In Situ Hybridization (FISH)
  • In Vitro Assessment of Human Natural Killer Cell Migration and Invasion
  • Microfluidic-Based Live-Cell Analysis of NK-Cell Migration In Vitro
  • Microwell-Based Live Cell Imaging of NK Cell Dynamics to Assess Heterogeneity in Motility and Cytotoxic Response
  • Assessment of Natural Killer Cell Cytotoxicity Using Image Cytometry Method
  • Analysis of Intracellular Ca2+ Mobilization in Human NK Cell Subsets by Flow Cytometry
  • Using NK Cell Lipid Raft Fractionation to Understand the Role of Lipid Rafts in NK Cell Receptor Signaling
  • High- and Super-Resolution Microscopy Imaging of the NK Cell Immunological Synapse
  • The Planar Lipid Bilayer System Serves as a Reductionist Approach for Studying NK Cell Immunological Synapses and their Functions
  • Expansion of NK Cells Using Genetically Engineered K562 Feeder Cells
  • Ex-Vivo Expansion of Human NK Cells Using K562 Engineered to Express Membrane Bound IL21
  • Large-Scale Culture and Genetic Modification of Human Natural Killer Cells for Cellular Therapy
  • Gene Modification of Human Natural Killer Cells Using a Retroviral Vector
  • Modification of Expanded NK Cells with Chimeric Antigen Receptor mRNA for Adoptive Cellular Therapy
  • mRNA Transfection to Improve NK Cell Homing to Tumors
  • In Vitro Generation of Human NK Cells Expressing Chimeric Antigen Receptor through Differentiation of Gene-Modified Hematopoietic Stem Cells
  • Engineering Receptor Expression on Natural Killer Cells through Trogocytosis
  • Electroporation of siRNA to Silence Gene Expression in Primary NK Cells
  • Mouse Xenograft Model for Intraperitoneal Administration of NK Cell Immunotherapy for Ovarian Cancer
  • Aerosol Delivery of Interleukin-2 in Combination with Adoptive Transfer of Natural Killer Cells for the Treatment of Lung Metastasis: Methodology and Effect
  • Non-Invasive Imaging of Natural Killer Cell-Mediated Apoptosis in a Mouse Tumor Model
  • Non-Invasive In Vivo Fluorescence Imaging of NK Cells in Pre-Clinical Models of Adoptive Immunotherapy
  • In Vivo 19F-Magnetic Resonance Imaging of Adoptively Transferred NK Cells
  • Generation of BiKEs and TriKEs to Improve NK Cell-Mediated Targeting of Tumor Cells
  • Regulatory Considerations for NK Cells Used in Human Immunotherapy Applications.