Human Respiratory Syncytial Virus Methods and Protocols - Tripp, Ralph A.; Jorquera, Patricia A.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2016)
Human Respiratory Syncytial Virus: An Introduction -- RSV Growth and Quantification by Microtitration and qRT-PCR Assays -- Quantification of RSV Infectious Particles by Plaque Assay and Immunostaining Assay -- Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays -- Secretory Expression and Purification of Respiratory Syncytial Virus G and F Proteins in Human Cells -- Development of Human Monoclonal Antibodies against Respiratory Syncytial Virus Using a High Efficiency Human Hybridoma Technique -- Respiratory Syncytial Virus (RSV): Neutralizing Antibody, a Correlate of Immune Protection -- Host Factors Modulating RSV Infection; Use of Small Interfering RNAs to Probe Functional Importance -- In Vitro Modelling of RSV Infection and Cytopathogenesis in Well-Differentiated Human Primary Airway Epithelial Cells (WD-PAECs) -- Reverse Genetics of Respiratory Syncytial Virus -- Use of Minigenome Systems to Study RSV Transcription -- Screening for Host Factors Directly Interacting with RSV Protein: Microfluidics -- A Proteomic-Based Workflow Using Purified Respiratory Syncytial Virus Particles to Identify Cellular Factors as Drug Targets -- MicroRNA Profiling from RSV Infected Biofluids, Whole Blood, and Tissue Samples -- Mouse and Cotton Rat Models of Human Respiratory Syncytial Virus -- In Vivo Assessment of Airway Function in the Mouse Model -- Evaluation of the Adaptive Immune Response to Respiratory Syncytial Virus. .
Keywords: Infectious diseases; Infectious Diseases