Hepatitis B Virus Methods and Protocols - Guo, Haitao; Cuconati, Andrea; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
NTCP-Reconstituted In Vitro HBV Infection System -- Hepatitis B Virus Infection of HepaRG Cells, HepaRG-hNTCP Cells and Primary Human Hepatocytes -- Live Cell Imaging Confocal Microscopy Analysis of HBV Myr-PreS1 Peptide Binding and Uptake in NTCP-GFP Expressing HepG2 Cells -- Intracytoplasmic Transport of Hepatitis B Virus Capsids -- A Homokaryon Assay for Nucleocytoplasmic Shuttling Activity of HBV Core Protein -- Analyses of HBV cccDNA Quantification and Modification -- Detection of HBV cccDNA Methylation from Clinical Samples by Bisulfite Sequencing and Methylation Specific PCR -- A T7 Endonuclease I Assay to Detect TALEN-Mediated Mutation of HBV cccDNA -- Detection of Hepatocyte Clones Containing Integrated Hepatitis B Virus DNA using Inverse Nested PCR -- Highly Sensitive Detection of HBV RNA in Liver Tissue by In Situ Hybridization -- Immunofluorescent Staining for the Detection of the Hepatitis B Core Antigen in Frozen Liver Sections of Human Liver Chimeric Mice -- Measuring Changes in Cytosolic Calcium Levels in HBV- and HBx-Expressing Cultured Primary Hepatocytes -- In Vitro Assays for RNA Binding and Protein Priming of Hepatitis B Virus Polymerase -- In Vitro Enzymatic and Cell Culture-Based Assays for Measuring Activity of HBV RNaseH Inhibitors -- Detection of Hepatitis B Virus Particles Released from Cultured Cells by Particle Gel Assay -- Microtiter-Format Assays for HBV Antigen Quantitation in Non-Clinical Applications -- Deep Sequencing of the Hepatitis B Virus Genome: Analysis of Multiple Samples by Implementation of the Illumina Platform -- Generation of Replication Competent Hepatitis B Virus Genome from Blood Samples for Functional Characterization -- Hydrodynamic HBV Transfection Mouse Model -- An ELISPOT-Based Assay to Measure HBV-Specific CD8+ T Cell Responses in Immunocompetent Mice -- Advanced Method for Isolation of Mouse Hepatocytes, Liver Sinusoidal Endothelial Cells and Kupffer Cells -- Partial Hepatectomy and Castration of HBV Transgenic Mice -- Studying HBV Infection and Therapy in Immune Deficient NOD-Rag1-/-IL2RgammaC-null (NRG) Fumarylacetoacetate Hydrolase (Fah) Knockout Mice Transplanted with Human Hepatocytes -- Measurement of Antiviral Effect and Innate Immune Response during Treatment of Primary Woodchuck Hepatocytes.
Keywords: Infectious diseases; Infectious Diseases