Mitochondria Practical Protocols - Mokranjac, Dejana; Perocchi, Fabiana; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
A Guide to Computational Methods for Predicting Mitochondrial Localization -- Isolation of Functional Mitochondria from Cultured Cells and Mouse Tissues -- Isolation of Mitochondria From Saccharomyces Cerevisiae -- Isolation of Contact Sites Between Inner and Outer Mitochondrial Membranes -- Isolation Of Mitochondria-Associated Membranes (MAM) from Mouse Brain Tissue -- Label-Free Quantitative Analysis of Mitochondrial Proteomes using the Multienzyme Digestion-Filter Aided Sample Preparation (MED-FASP) and 'Total Protein Approach' -- Quantitative Analysis of Glycerophospholipids in Mitochondria by Mass Spectrometry -- Detection of Cysteine Redox States in Mitochondrial Proteins in Intact Mammalian Cells -- Chemical Crosslinking in Intact Mitochondria -- Reconstitution of Mitochondrial Membrane Proteins into Nanodiscs by Cell-Free Expression -- Detection of Dual Targeting and Dual Function of Mitochondrial Proteins in Yeast -- Localizing Mrnas Encoding Mitochondrial Proteins in Yeast by Fluorescence Microscopy and Subcellular Fractionation -- Assessing Mitochondrial Bioenergetics in Isolated Mitochondria from Various Mouse Tissues using Seahorse XF96 Analyzer -- Application of FRET-Based Biosensor "Ateam" for Visualization of ATP Levels in the Mitochondrial Matrix of Living Mammalian Cells -- A Microplate-Based Bioluminescence Assay of Mitochondrial Calcium Uptake -- New Imaging Tools to Analyze Mitochondrial Morphology in Caenorhabditis Elegans -- Single Molecule Tracking and Localization Of Mitochondrial Protein Complexes in Live Cells -- Analysis of Yeast Mitochondria by Electron Microscopy -- Analysis of Mitochondrial Membrane Protein Complexes by Electron Cryo-Tomography -- Assays for Mitophagy in Yeast -- Assessing Mitochondrial Selective Autophagy in the Nematode Caenorhabditis Elegans -- Assessing Mitochondrial Unfolded Protein Response in Mammalian Cells -- Analysis of Mitochondrial RNA-Processing Defects in Patient-Derived Tissues by Qrt-PCR and Rnaseq -- Identification of Disease-Causing Mutations by Functional Complementation of Patient-Derived Fibroblast Cell Lines. .
Keywords: Cell biology; Cell Biology