Heterologous Gene Expression in E.coli Methods and Protocols - Burgess-Brown, Nicola A.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
Recombinant Protein Expression in E. coli: A Historical Perspective -- N- and C-Terminal Truncations to Enhance Protein Solubility and Crystallization: Predicting Protein Domain Boundaries with Bioinformatics Tools -- Harnessing the Profinity eXactTM System for Expression and Purification of Heterologous Proteins in E. coli -- ESPRIT: A Method for Defining Soluble Expression Constructs in Poorly Understood Gene Sequences -- Optimizing Expression and Solubility of Proteins in E. coli Using Modified Media and Induction Parameters -- Optimization of Membrane Protein Production Using Titratable Strains of E. coli -- Optimizing E. coli-Based Membrane Protein Production Using Lemo21(DE3) or pReX and GFP-Fusions -- High Yield of Recombinant Protein in Shaken E. coli Cultures with Enzymatic Glucose Release Medium EnPresso B -- A Generic Protocol for Purifying Disulphide-Bonded Domains and Random Protein Fragments Using Fusion Proteins with SUMO3 and Cleavage by SenP2 Protease -- A Strategy for Production of Correctly Folded Disulfide-Rich Peptides in the Periplasm of E. coli -- Split GFP Complementation as Reporter of Membrane Protein Expression and Stability in E. coli: A Tool to Engineer Stability in a LAT Transporter -- Acting on Folding Effectors to Improve Recombinant Protein Yields and Functional Quality -- Protein Folding Using a Vortex Fluidic Device -- Removal of Affinity Tags with TEV Protease -- Generation of Recombinant N-Linked Glycoproteins in E. coli -- Production of Protein Kinases in E. coli -- Expression of Prokaryotic Integral Membrane Proteins in E. coli -- Multiprotein Complex Production in E. coli: The SecYEG-SecDFYajC-YidC Holotranslocon -- Membrane Protein Production in E. coli Lysates in Presence of Pre-Assembled Nanodiscs -- Not Limited to E. coli: Versatile Expression Vectors for Mammalian Protein Expression -- A Generic Protocol for Intracellular Expression of Recombinant Proteins in Bacillus subtilis -- In Vivo Biotinylation of Antigens in E. coli -- Cold-Shock Expression System in E. coli for Protein NMR Studies -- High-Throughput Production of Proteins in E. coli for Structural Studies -- Mass Spectrometric Analysis of Proteins -- How to Determine Interdependencies of Glucose and Lactose Uptake Rates for Heterologous Protein Production with E. coli -- Interfacing Biocompatible Reactions with Engineered Escherichia coli.
Keywords: Microbiology; Microbiology