Membrane Protein Structure and Function Characterization Methods and Protocols - Lacapere, Jean-Jacques.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
Recombinant Overexpression of Mammalian TSPO Isoforms 1 and 2 -- Functional Assembly of Soluble and Membrane Recombinant Proteins of Mammalian NADPH Oxidase Complex -- Direct Extraction and Purification of Recombinant Membrane Proteins from Pichia pastoris protoplasts -- Cell-free Expression for the Study of Hydrophobic Proteins: The Example of Yeast ATP-synthase subunits -- Wheat Germ Cell Free Overexpression for the Production of Membrane Proteins -- Methyl-specific Isotope Labelling Strategies for NMR Studies of Membrane Proteins -- Labeling of Membrane Complexes for Electron Microscopy -- Expression, Biochemistry, and Stabilization with Camel Antibodies of Membrane Proteins Case Study of The Mouse 5-HT Receptor -- Characterization of New Detergent and Detergent Mimetics by Scattering Techniques for Membrane Protein Crystallization -- Secondary Structure Determination by Means of ATR-FTIR Spectroscopy -- Native Mass Spectroscopy for the Characterization of Structure and Interactions of Membrane Proteins -- Mass Spectroscopy of Mitochondrial Membrane Proteins Complexes -- Functional Studies on Membrane Proteins by Means of H/D Exchange in Infrared: Structural Changes in Na+NQR from V cholerae in the Presence of Lipids -- Reconstitution of Membrane Protein in Liposomes -- Ion Channels as Reporters of Membrane Receptor Function: Automated Analysis in Xenopus Oocytes -- The CRACAM Robot: Two Dimensional Crystallization of Membrane Protein -- Reconstitution of Membrane Proteins into Nanodiscs for Single Particle Electron Microscopy -- Solid-state NMR of Membrane Protein Reconstituted in Proteoliposomes, the Case of TSPO -- Sample Preparation for Membrane Protein Structural Studies by Solid-State NMR -- Simulation of Ligand Binding To Membrane Proteins -- Molecular modelling of Transporters: From Low Resolution Cryo-Electron Microscopy Map to Conformational Exploration. The Example of TSPO Molecular Dynamic of Membrane Protein in Different Environments. .
Keywords: Proteins .; Protein Science