Plant Argonaute Proteins Methods and Protocols - Carbonell, Alberto; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
Plant ARGONAUTEs: Features, Functions and Unknowns -- Analysis of the Uridylation of Both ARGONAUTE-Bound MiRNAs and 5' Cleavage Products of their Target RNAs in Plants -- In Vitro Formation of Plant RNA-Induced Silencing Complexes Using an Extract of Evacuolated Tobacco Protoplasts -- In vitro Analysis of ARGONAUTE-Mediated Target Cleavage and Translational Repression in Plants -- Establishment of an In Vivo ARGONAUTE Reporter System in Plants -- Immunoprecipitation and High-throughput Sequencing of ARGONAUTE-Bound RNAs from Plants -- Identification of ARGONAUTE/Small RNA Cleavage Sites by Degradome Sequencing -- Dissecting the Sub-Nuclear Localization Patterns of ARGONAUTE Proteins and Other Components of the RNA-Directed DNA Methylation Pathway in Plants -- Detection of ARGONAUTE 1 Association with Polysomes in Arabidopsis thaliana -- Functional Analysis of Arabidopsis ARGONAUTEs in Meiosis and DNA Repair -- Isolation and Characterization of ARGONAUTE Mutants in Chlamydomonas -- ARGONAUTE genes in Salvia miltiorrhiza: Identification, Characterization and Genetic Transformation -- Cloning and Characterization of ARGONAUTE Genes in Tomato -- Non-Radioactive Detection of Small RNAs Using Digoxygenin-Labeled Probes -- Non-Radioactive Plant Small RNA Detection Using Biotin-Labeled Probes -- Computational Analysis of Genome-Wide ARGONAUTE-Dependent DNA -- Structural and Functional Characterization of Plant ARGONAUTE MID Domains -- Identification and Analysis of WG/GW ARGONAUTE-Binding Domains -- Identification and Evolutionary Characterization of ARGONAUTE-Binding Platforms -- Phylogenetic and Evolutionary Analysis of Plant ARGONAUTES.
Keywords: Plant science; Botany; Plant Sciences