Antigen Processing Methods and Protocols - van Endert, Peter; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2019)
Purification of Large Cytosolic Proteases for In Vitro Assays: 20S and 26S Proteasomes -- Analysis of Proteasome Generated Antigenic Peptides by Mass Spectrometry -- Trimming of MHC Class I Ligands by ERAP Aminopeptidases -- Peptide Trimming for MHC Class I Presentation by Endoplasmic Reticulum Aminopeptidases -- Testing the Impact of Protease Inhibitors in Antigen Presentation Assays -- In Vitro Studies of MHC Class I Peptide Loading and Exchange -- Measuring Synthesis and Degradation of MHC Class I Molecules -- Biochemical Analysis of Naturally Processed Antigenic Peptides Presented by MHC Class I Molecules -- A SIINFEKL Based System to Measure MHC Class I Antigen Presentation Efficiency and Kinetics -- Purification and Identification of Naturally Presented MHC Class I and II Ligands -- Identification of MHC Ligands and Establishing MHC Class I Peptide Motifs -- Quantitating MHC Class I Ligand Production and Presentation using TCR-Like Antibodies -- Identifying Antigens Recognized by Cytolytic T Lymphocytes on Tumors -- A Flow Cytometry-Based Approach to Unravel Viral Interference with the MHC Class I Antigen Processing and Presentation Pathway -- Evaluating CD8+ T Cell Responses In Vitro -- Recombinant Poxviruses: Versatile Tools for Immunological Assays -- Monitoring the Intracellular Routing of Internalized Antigens by Immunofluorescence Microscopy -- Monitoring MHC Ubiquitination by MARCH Ubiquitin Ligases -- Monitoring MHC-II Endocytosis and Recycling using Cell-Surface Protein Biotinylation-Based Assays -- Monitoring Protein Endocytosis and Recycling using FACS-Based Assays -- In Vitro Digestion with Proteases Producing MHC Class II Ligands -- Assembly, Intracellular Transport and Release of MHC Class II Peptide Receptors -- Pulse-Chase Analysis for Studies of MHC Class II Biosynthesis, Maturation, and Peptide Loading -- Studying MHC Class II Peptide Loading and Editing In Vitro -- Monitoring Antigen Processing for MHC Presentation via Macroautophagy -- Identification of Human Antigen-Specific CD4+ T-Cells with Peptide-MHC Multimer Technologies -- Production of Antigen Specific Human CD4+ T Cell Lines and Clones -- Monitoring Dendritic Cell Activation and Maturation -- Studying MHC Class II Presentation of Immobilized Antigen by B Lymphocytes -- Analyzing Antigen Recognition by Natural Killer T Cells.
Keywords: Molecular biology; Antibodies; Molecular Medicine; Antibodies