Antiviral Resistance in Plants Methods and Protocols - Kobayashi, Kappei; Nishiguchi, Masamichi; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2019)
Identification and Functional Analysis of NB-LRR-Type Virus Resistance Genes-Overview and Functional Analysis of Candidate Genes -- Insertion of Epitope Tag into NB-LRR Class Plant Virus Resistance Protein -- Reverse genetic analysis of antiviral resistance signaling and the resistance mechanism in Arabidopsis thaliana -- Analysis of Antiviral Resistance Signaling Pathways by Virus-Induced Gene Silencing in Nicotiana benthamiana -- Random Mutagenesis of Virus Gene for the Experimental Evaluation of the Durability of NB-LRR Class Plant Virus Resistance Gene -- A Cell-free Replication System for Positive-strand RNA Viruses for Identification and Characterization of Plant Resistance Gene Products -- Plant Protein-Mediated Inhibition of Virus Cell-to-Cell Movement -Far-western Screening and Biological Analysis of a Plant Protein Interacting with a Viral Movement Protein- -- Transfection of Protoplasts Prepared from Arabidopsis thaliana Leaves for Plant Virus Research -- CRISPR/Cas9-mediated Editing of Genes Encoding rgs-CaM-like Proteins in Transgenic Potato Plants -- A Simplified Method to Engineer CRISPR/Cas9-Mediated Geminivirus Resistance in Plants -- Computational Workflow for Small RNA Profiling in Virus-Infected Plants -- A Sensitized Genetic Screen to Identify Novel Components and Regulators of the Host Antiviral RNA Interference Pathway -- Design, Synthesis and Functional Analysis of Highly Specific Artificial Small RNAs With Antiviral Activity in Plants -- Resistance Breeding through RNA Silencing of Host Factor Involved in Virus Replication -- Large-Scale Inoculation and Evaluation Methods for Attenuated Plant Viruses -- RNA Silencing-Mediated Apple Latent Spherical Virus Vaccine in Plants.
Keywords: Plant science; Botany; Virology; Plant Sciences; Virology