Cryptosporidium Methods and Protocols - Mead, Jan R.; Arrowood, Michael J.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2020)
Cryptosporidium Diagnostic Assays: Microscopy -- Cryptosporidium Diagnostic Assays: Molecular Detection -- Detection of Cryptosporidium Recovered From Large-Volume Water Samples Using Dead-End Ultrafiltration -- Cryptosporidium Oocyst Purification Using Discontinuous Gradient Centrifugation -- Measuring Cryptosporidium Serologic Responses by Multiplex Bead Assay -- Production and Purification of Functional Cryptosporidium Glycoproteins by Heterologous Expression in Toxoplasma gondii -- Cryptosporidium Genotyping for Epidemiology Tracking -- Use of Markers to Determine Cryptosporidium Genotypes for Epidemiology Tracking and Detection -- Preparation of Cryptosporidium DNA for Whole Genome Sequencing -- Accessing Cryptosporidium Omic and Isolate Data Via CryptoDB.org -- A Novel Method to Silence Genes in Cryptosporidium -- Use of miRNAs to Study Host Cell-Parasite Interactions -- Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9 -- Mouse Models for Use in Cryptosporidium Infection Studies and Quantification of Parasite Burden Using Flow Cytometry, qPCR, and Histopathology -- Calf Clinical Model of Cryptosporidiosis for Efficacy Evaluation of Therapeutics -- Cell Culture Infectivity to Assess Chlorine Disinfection of Cryptosporidium Oocysts in Water -- High-Content Screening for Cryptosporidium Drug Discovery -- High-Throughput Screening of Drugs Against the Growth of Cryptosporidium parvum In Vitro by qRT-PCR -- In Vitro Culture of Cryptosporidium parvum Using Hollow Fiber Bioreactor: Applications for Simultaneous Pharmacokinetic and Pharmacodynamic Evaluation of Test Compounds -- In Vitro Culture of Cryptosporidium parvum Using Stem Cell-Derived Intestinal Epithelial Monolayers -- Two and Three Dimensional Bioengineered Human Intestinal Tissue Models for Cryptosporidium.
Keywords: Parasitology; Microbiology; Infectious diseases; Parasitology; Microbiology; Infectious Diseases