Matrix Metalloproteinase Protocols - Clark, Ian M.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2001)
MMPs and TIMPs-An Overview of the Field -- MMPs and TIMPs -- Strategies for Cloning New MMPs and TIMPs -- Structural Studies on MMPs andTIMPs Wolfram Bode and Klaus Maskos -- Matrix MetaIIoproteinase Substrate Binding Domains, Modules and Exosites -- The Matrix Metalloproteinase (MMP) and Tissue Inhibitor of Metalloproteinase (TIMP) Genes -- Models for Gain-of-Function and Loss-of-Function of MMPs -- Expression and Purification of MMPs and TIMPs -- Expression of MMPs andTIMPs in Mammalian Cells -- Expression of Recombinant Matrix Metalloproteinases in Escherichia coli -- Expression of Human Collagenase I (MMP-1) andTIMP-1 in a Baculovirus-Based Expression System -- Expression of Recombinant Matrix Metalloproteinases in Yeast -- Expression of Recombinant Membrane-Type MMPs -- Refolding of TIMP-2 from Escherichia coli Inclusion Bodies -- Expression and Refolding of Full-Length HumanTIMP-1 -- Purification of MMPs and TIMPs -- Detection of MMPs and TIMPs -- Monitoring MMP andTIMP mRNA Expression by RT-PCR -- Measuring Transcription of Metalloproteinase Genes -- In Situ Hybridization for Metalloproteinases and Their Inhibitors -- Use of EIA to Measure MMPs and TIMPs -- Immunohistochemistry of MMPs and TIMPs -- Detecting Polymorphisms in MMP Genes -- Assay of MMP and TIMP Activities -- Methods for Studying Activation of Matrix Metalloproteinases -- Assay of Matrix Metalloproteinases Against Matrix Substrates -- Zymography and Reverse Zymography for Detecting MMPs, andTIMPs -- In Situ Zymography -- Detection of Focal Proteolysis Using Texas-Red-Gelatin -- Antibodies to MMP-Cleaved Aggrecan -- Cartilage Proteoglycan Release Assay -- Immunoassay for Collagenase-Mediated Cleavage of Types I and II Collagens -- Collagen Degradation Assays -- Invasion Assays and Matrix Metalloproteinases -- Using Fluorogenic Peptide Substrates to Assay Matrix Metalloproteinases -- Kinetic Analysis of the Inhibition of Matrix Metalloproteinases by Tissue Inhibitor of Metalloproteinases (TIMP) -- Assaying Growth Factor Activity of Tissue Inhibitors of Metalloproteinases.
Keywords: Biochemistry; Biochemistry, general