Transforming Growth Factor-Beta Protocols - Howe, Philip H.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2000)
In Vitro Assays for Measuring TGF? Growth Stimulation and Inhibition -- Measurements of Active TGF? Generated by Culture Cells -- Methods for Iodination of Active TGF?, TGF? Receptor Crosslinking and Immunoprecipitation of TGF?-Receptor Complexes -- Detection of TGF? in Body Fluids and Tissues -- Analysis of TGF?-Mediated Synthesis of Extracellular Matrix Components -- Characterization of SMAD Phosphorylation and SMAD/Receptor Interaction -- Promoter Analysis of TGF?-Responsive Genes by Transient Transfection and Deletional/Mutational Analysis -- Regulation of AP-1 Activity by TGF? -- CDK Pathway: Cyclin-Dependent Kinases (cdks) and Cyclin-Dependent Kinase Inhibitors -- Activation of the Mitogen-Activated Protein Kinase Pathway by TGF? -- 11 Detection of TGF? Type II Receptor Hotspot Mutations: The BAT-RII Assay -- Mutation Detection in the TGF? Receptors and SMAD Genes: RT-PCR and Sequencing -- Analysis of TGF?-Inducible Apoptosis.
Keywords: Cell biology; Cell Biology