Protein Kinase Protocols - Reith, Alastair D.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2001)
Protein Kinase-Mediated Signaling Networks -- Cloning Protein Tyrosine Kinases by Screening cDNA Libraries with Antiphosphotyrosine Antibodies -- Immunoprecipitation and Western Blotting of Phosphotyrosine-Containing Proteins -- Two-Dimensional Phosphoamino Acid Analysis -- Two-Dimensional Phosphopeptide Mapping of Receptor Tyrosine Kinases -- Identification of the Sites of Phosphorylation in Proteins Using High Performance Liquid Chromatography and Mass Spectrometry -- Phosphorylation of Smad Signaling Proteins by Receptor Serine/Threonine Kinases -- Assays for Mitogen-Activated Protein Kinase (MARK) Subtypes and MARK Activating Protein Kinase-2 (MAPKAP K-2) Using a Common Cell Lysate -- JAK-Mediated Phosphorylation and Activation of STAT Signaling Proteins -- Assays for Glycogen Synthase Kinase-3(GSK-3) -- Cyclin-Dependent Kinases and Cyclin-Dependent Kinase Inhibitors -- Protein Histidine Kinase -- Cloning and Expression of Recombinant HEXA-HIS Tagged ZAP-70 Using the Baculovirus System -- Analysis of Protein Kinase Subcellular Localization by Visualization of GFP Fusion Proteins -- Detection of Phosphorylation-Dependent Interactions by Far-Western Gel Overlay -- Purification of Tyrosine-Phosphorylated Proteins by Immunoaffinity Chromatography and Direct Cloning of Their cDNAs from Bacterial Expression Libraries -- Identification of Receptor Tyrosine Kinase (RTK) Substrates by the Cloning of ReceptorTargets (CORT) Strategy -- Identification of Receptor Tyrosine Kinase Associating Proteins Using the Yeast Two-Hybrid System -- cDNA Expression Cloning and Characterization of Phosphorylation Dependent Protein Interactors Using the Yeast Tribrid System -- Analysis of SH2 Domain - Phosphopeptide Interactions by Isothermal Titration Calorimetry and Surface Plasmon Resonance -- Cloning and Characterization of RTK Ligands Using Receptor-Alkaline Phosphatase Fusion Proteins -- Isolation and Characterization of "Orphan-RTK" Ligands Using an Integrated Biosensor Approach.
Keywords: Biochemistry; Biochemistry, general