Neurotrophin Protocols - Rush, Robert A.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2001)
Neurotrophin Quantification and Localization -- Localization of Neurotrophin Proteins Within the Central Nervous System by Immunohistochemistry -- Neurotrophin Immunohistochemistry in Peripheral Tissues -- Extraction and Quantification of the Neurotrophins -- Identifying Novel Proteins in Nervous Tissue Using Microsequencing Techniques -- RNA Quantification and Localization -- Quantification of mRNA Levels Using Northern Blotting -- Quantification of mRNA Levels Using Ribonuclease Protection Assay -- Quantification of Neurotrophin mRNA by RT-PCR -- Sensitive and Nonradioactive In Situ Detection of Neurotrophin mRNAs in the Nervous System -- Construction of Recombinant Proteins,and Transgenic Cells and Animals -- Expression of Neurotrophic Factors such as GDNF in the E. coli System -- Construction of Cells Expressing Neurotrophins -- Efficient Generation of Stable Pheochromocytoma (PC12) Cell Lines Using a Recombinant Retrovirus (LNC) -- Construction and Analysis of Transgenic Animals Overexpressing Neurotrophins -- Construction of Transgenic Animals Overproducing Neurotrophins and Their Receptors -- In Vivo Analysis -- Tracing with Radiolabeled Neurotrophins -- Toxin-Induced Death of Neurotrophin-Sensitive Neurons -- Oligonucleotides as Inhibitors of Protein Synthesis -- The IODO-GEN Method for Labeling and the Use of Retrograde Axonal Transport to Assay Neurotrophins -- Stereological Estimation of Neuronal Number The Optical Disector/Cavalieri Combination.
Keywords: Neurosciences; Neurosciences