Kinesin Protocols - Vernos, Isabelle; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2001)
Purification of Kinesin from the Brain -- RT-PCR for the Identification of Developmentally Regulated Novel Members of the Kinesin-like Superfamily -- Expression Cloning with Pan Kinesin Antibodies -- Expression of Kinesin in Escherichia coli -- Plasmids for Expression of Chimeric and Truncated Kinesin Proteins -- Preparation of Recombinant Kinesin Superfamily Proteins Using the Baculovirus System -- Assays for Kinesin Microtubule-Stimulated AT Pase Activity -- An Improved Microscope for Bead and Surface-Based Motility Assays -- Use of Photonic Force Microscopy to Study Single-Motor-Molecule Mechanics -- Assays for Microtubule-Destabilizing Kinesins -- Green Fluorescent Protein as a Tag for Molecular Motor Proteins -- In Vitro Reconstitution of Endosome Motility Along Microtubules -- Approaches to Study Interactions Between Kinesin Motors and Membranes -- Microinjection Methods for Analyzing the Functions of Kinesins in Early Embryos -- The Use of Dominant Negative Mutants to Study the Function of Mitotic Motors in the In Vitro Spindle Assembly Assay in Xenopus Egg Extracts -- A Dominant Negative Approach for Functional Studies of the Kinesin II Complex -- Identification of Kinesin-Associated Proteins -- Assaying Spatial Organization of Microtubules by Kinesin Motors -- Crystallization of Kinesin -- Structural Analysis of the Microtubule-Kinesin Complex by Cryo-Electron Microscopy.
Keywords: Cell biology; Cell Biology