Steroid Receptor Methods Protocols and Assays - Lieberman, Benjamin A.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2001)
Bioinformatics -- Bioinformatics of Nuclear Receptors -- Phylogenetic Inference and Parsimony Analysis -- Purification Protocols -- Expression and Purification of Recombinant Human Progesterone Receptor in Baculovirus and Bacterial Systems -- High-Yield Purification of Functionally Active Glucocorticoid Receptor -- Production and Purification of Histidine-Tagged Dihydrotestosterone-Bound Full-Length Human Androgen Receptor -- Large Scale Production of Nuclear Receptor Ligand-Binding Domains -- Physical Structure of Nuclear Receptor-DNA Complexes -- Isolation of Steroid-Regulated Genes from the Uterus by mRNA Differential Display -- Identification of Nuclear Hormone Receptor Homologs by Screening Libraries with Highly Degenerate Oligonucleotide Probes -- Steroid Hormone-Binding Assays -- Use of [99mTc]Technetium-Labeled Steroids as Probes for Steroid Hormone Receptors -- Steroid Hormone Metabolites and Hormone Binding Assays -- Vitamin D3 Analog Screening -- Protein Interaction Assays -- Application of Green Fluorescent Protein to the Study of Dynamic Protein-Protein Interactions and Subcellular Trafficking of Steroid Receptors -- Knockout Mice and Steroid Receptor Research -- Yeast Two-Hybrid Screening for Proteins that Interact with Nuclear Hormone Receptors -- Isolation of a p300/CBP Cointegrator-Associated Protein Coactivator Complex -- Nonradioactive Photoaffinity Labeling of Steroid Receptors Using Western Blot Detection System -- Analysis of Steroid Hormone-Induced Histone Acetylation by Chromatin Immunoprecipitation Assay -- Analyzing the Contributions of Chromatin Structure in Nuclear Hormone Receptor Activated Transcription In Vivo -- Cotransfection Assays and Steroid Receptor Biology -- Estrogen Receptor mRNA In Situ Hybridization Using Microprobe System -- Cancer Research and Drug Discovery -- Solid Tumor Cancer Markers and Applications to Steroid Hormone Research -- Assessing Modulation of Estrogenic Activity of Environmental and Pharmaceutical Compounds Using MCF-7 Focus Assay -- Combinatorial Chemistry in Steroid Receptor Drug Discovery -- Identification of Nuclear Receptor Interacting Proteins Using Yeast Two- Hybrid Technology.
Keywords: Biochemistry; Biochemistry, general