Mycobacterium Tuberculosis Protocols - Parish, Tanya; Stoker, Neil G.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2001)
Plasmid Vectors -- Isolation of DNA from Mycobacterium tubercolosis -- Extraction of RNA from Intracellular Mycobacterium tuberculosis -- Transposon Mutagenesis in Mycobacteria Using Conditionally Replicating Mycobacteriophages -- Gene Replacement and Transposon Delivery Using the Negative Selection Marker sacB -- Gene Replacement using Pretreated DNA -- Gene Replacement in Mycobacterium tuberculosis and Mycobacterium bovis BCG Using rpsL as a Dominant Negative Selectable Marker -- Transcription Start-Site Mapping -- Fluorescence and Brightfield Cytology of Live M. tuberculosis Cells -- Phage Replication Technology for Diagnosis and Drug Susceptibility Testing -- Detection of Mutations in Mycobacterium tuberculosis by a Dot Blot Hybridization Strategy -- Restriction Fragment Length Polymorphism Typing of Mycobacteria -- Preparation of Culture Filtrate Proteins from Mycobacterium tuberculosis -- Analysis of the Capsule of Mycobacterium tuberculosis -- Analysis of the Lipids of Mycobacterium tuberculosis -- In Vitro Model of Hypoxically Induced Nonreplicating Persistence of Mycobacterium tuberculosis -- 17 Macrophage Virulence Assays -- Analysis of Mycobacterium-Infected Macrophages by Immunoelectron Microscopy and Cell Fractionation -- Real Time PCR Using Molecular Beacons -- Electronic Access to Mycobacterium tuberculosis Sequence Data -- Proteomics -- Functional Genomics of Mycobacterium tuberculosis Using DNA Microarrays -- Storage of Mycobacterial Strains -- Safety in the Laboratory.
Keywords: Infectious diseases; Infectious Diseases