PCR Cloning Protocols - Chen, Bing-Yuan.; Janes, Harry W.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2002)
Performing and Optimizing PCR -- Polymerase Chain Reaction -- Computer Programs for PCR Primer Design and Analysis -- Single-Step PCR Optimization Using Touchdown and Stepdown PCR Programming -- XL PCR Amplification of Long Targets from Genomic DNA -- Coupled One-Step Reverse Transcription and Polymerase Chain Reaction Procedure for Cloning Large cDNA Fragments -- Long Distance Reverse-Transcription PCR -- Increasing PCR Sensitivity for Amplification from Paraffin-Embedded Tissues -- GC-Rich Template Amplification by Inverse PCR -- PCR Procedure for the Isolation of Trinucleotide Repeats -- Methylation-Specific PCR -- Direct Cloning of Full-Length Cell Differentially Expressed Genes by Multiple Rounds of Subtractive Hybridization Based on Long-Distance PCR and Magnetic Beads -- Cloning PCR Products -- Cloning PCR Products -- Using T4 DNA Polymerase to Generate Clonable PCR Products -- Enzyme-Free Cloning of PCR Products and Fusion Protein Expression -- Directional Restriction Site-Free Insertion of PCR Products into Vectors -- Autosticky PCR -- A Rapid and Simple Procedure for Direct Cloning of PCR Products into Baculoviruses -- Mutagenesis and Recombination -- PCR Approaches to DNA Mutagenesis and Recombination -- In-Frame Cloning of Synthetic Genes Using PCR Inserts -- Megaprimer PCR -- PCR-Mediated Recombination -- PCR Method for Generating Multiple Mutations at Adjacent Sites -- A Fast Polymerase Chain Reaction-Mediated Strategy for Introducing Repeat Expansions into CAG-Repeat Containing Genes -- PCR Screening in Signature-Tagged Mutagenesis of Essential Genes -- Staggered Extension Process (StEP) In Vitro Recombination -- Random Mutagenesis by Whole-Plasmid PCR Amplification -- Cloning Unknown Neighboring DNA -- PCR-Based Strategies to Clone Unknown DNA Regions from Known Foreign Integrants -- Long Distance Vectorette PCR (LDV PCR) -- Nonspecific, Nested Suppression PCR Method for Isolation of Unknown Flanking DNA ("Cold-Start Method") -- Inverse PCR -- 31 Inverse PCR -- Gene Cloning and Expression Profiling by Rapid Amplification of Gene Inserts with Universal Vector Primers -- The Isolation of DNA Sequences Flanking Tn5 Transposon Insertions by Inverse PCR -- Rapid Amplification of Genomic DNA Sequences Tagged by Insertional Mutagenesis -- Isolation of Large-Terminal Sequences of BAC Inserts Based on Double-Restriction-Enzyme Digestion Followed by Anchored PCR -- A #x201C;Step Down#x201D; PCR-Based Technique for Walking Into and the Subsequent Direct-Sequence Analysis of Flanking Genomic DNA -- Library Construction and Screening -- Use of PCR in Library Screening -- Cloning of Homologous Genes by Gene-Capture PCR -- Rapid and Nonradioactive Screening of Recombinant Libraries by PCR -- Rapid cDNA Cloning by PCR Screening (RC-PCR) -- Generation and PCR Screening of Bacteriophage ? Sublibraries Enriched for Rare Clones (the "Sublibrary Method ") -- PCR-Based Screening for Bacterial Artificial Chromosome Libraries -- A 384-Well Microtiter-Plate-Based Template Preparation and Sequencing Method -- A Microtiter-Plate-Based High Throughput PCR Product Purification Method.
Keywords: Biochemistry; Cell biology; Biochemistry, general; Cell Biology; Science, Humanities and Social Sciences, multidisciplinary