Proteoglycan Protocols - Iozzo, Renato V.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2001)
Isolation and Purification -- Isolation of Proteoglycans from Cell Cultures and Tissues -- Isolation of Proteoglycans from Tendon -- Purification of Proteoglycans from Mineralized Tissues -- Purification of Perlecan from Endothelial Cells -- Isolation and Characterization of Nervous Tissue Proteoglycans -- Analysis of Proteoglycans and Glycosaminoglycans from Drosophila -- Cartilage and Smooth Muscle Cell Proteoglycans Detected by Affinity Blotting Using Biotinylated Hyaluronan -- Preparation of Proteoglycans for N-Terminal and Internal Amino Acid Sequence Analysis -- High-Specific-Activity 35S-Labeled Heparan Sulfate Prepared from Cultured Cells -- Selective Detection of Sulfotransferase Isoforms by the Ligand Affinity-Conversion Approach -- Particulate and Soluble Glycosaminoglycan-Synthesizing Enzymes -- Disaccharide Composition of Hyaluronan and Chondroitin/Dermatan Sulfate -- Integral Glycan Sequencing of Heparan Sulfate and Heparin Saccharides -- Analytical and Preparative Strong Anion-Exchange HPLC of Heparan Sulfate and Heparin Saccharides -- Proteoglycans Analyzed by Composite Gel Electrophoresis and Immunoblotting -- Quantitation of Proteoglycans in Biological Fluids Using Alcian Blue -- Cellulose Acetate Electrophoresis of Glycosaminoglycans -- Disaccharide Composition in Glycosaminoglycans/Proteoglycans Analyzed by Capillary Zone Electrophoresis -- Intact and Oligomeric Glycosaminoglycans Analyzed with Capillary Electrophoresis -- Expression, Detection, and Degradation -- Recombinant Expression of Proteoglycans in Mammalian Cells -- Purification of Recombinant Human Decorin and Its Subdomains -- Prokaryotic Expression of Proteoglycans -- Proteoglycan Gene Targeting in Somatic Cells -- Constitutive and Inducible Antisense Expression -- Cell-Mediated Transfer of Proteoglycan Genes -- Morphological Evaluation of Proteoglycans in Cells and Tissues -- Expression and Characterization of Engineered Proteoglycans -- Intracellular Localization of Engineered Proteoglycans -- Selection of Glycosaminoglycan-Deficient Mutants -- Xyloside Priming of Glycosaminoglycan Biosynthesis and Inhibition of Proteoglycan Assembly -- Inhibition of Heparan Sulfate Synthesis by Chlorate -- Detection of Proteoglycan Core Proteins with Glycosaminoglycan Lyases and Antibodies -- Proteoglycan Core Proteins and Catabolic Fragments Present in Tissues and Fluids -- Degradation of Heparan Sulfate by Nitrous Acid -- Degradation of Heparan Sulfate with Heparin Lyases -- Degradation of Chondroitin Sulfate and Dermatan Sulfate with Chondroitin Lyases -- In Vitro Assay for Hyaluronan Synthase -- Hyaluronidase Activity and Hyaluronidase Inhibitors -- Detecting Hyaluronidase and Hyaluronidase Inhibitors -- Interacations -- Affinity Coelectrophoresis of Proteoglycan-Protein Complexes -- Binding Constant Measurements for Inhibitors of Growth Factor Binding to Heparan Sulfate Proteoglycans -- Interaction of Proteoglycans with Receptor Tyrosine Kinases -- Measuring Single-Cell Cytosolic Ca2+ Concentration in Response to Proteoglycans -- Serum Amyloid A Peptide Interactions with Glycosaminoglycans -- Interactions of Lipoproteins with Proteoglycans -- Hyaluronan and Hyaluronan-Binding Proteins Probes for Specific Detection -- Novel Confocal-FRAP Analysis of Carbohydrate-Protein Interactions Within the Extracellular Matrix -- Regulatory Roles of Syndecans in Cell Adhesion and Invasion -- Optical Biosensor Techniques to Analyze Protein-Polysaccharide Interactions -- Phage Display Technology to Obtain Antiheparan Sulfate Antibodies -- Tissue-Specific Binding by FGF and FGF Receptors to Endogenous Heparan Sulfates.
Keywords: Biochemistry; Biochemistry, general