Two-Hybrid Systems Methods and Protocols - MacDonald, Paul N.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2001)
General Methods -- The Two-Hybrid System -- Growth and Maintenance of Yeast -- Media Formulations for Various Two-Hybrid Systems -- Yeast Two-Hybrid Vectors and Strains -- High-Efficiency Transformation of Plasmid DNA into Yeast -- Qualitative and Quantitative Assessment of Interactions -- Strategies for Rescuing Plasmid DNA from Yeast Two-Hybrid Colonies -- False Positives -- Two-Hybrid System and False Positives -- Confirming Yeast Two-Hybrid Protein Interactions Using In Vitro Glutathione-S-Transferase Pulldowns -- Two-Hybrid Interactions Confirmed by Coimmunoprecipitation of Epitope-Tagged Clones -- Applications -- Smad Interactors in Bone Morphogenetic Protein Signaling -- Protein Interactions Important in Eukaryotic Translation Initiation -- Steroid Receptor and Ligand-Dependent Interaction with Coactivator Proteins -- Interaction of Cellular Apoptosis Regulating Proteins with Adenovirus Anti-apoptosis Protein E1B-19K -- Alternative Strategies -- Mammalian Two-Hybrid Assays -- One-Hybrid Systems f hor Detecting Protein-DNA Interactions -- The Split-Hybrid System -- Three-Hybrid Screens -- Three-Hybrid Screens for RNA-Binding Proteins -- Membrane Recruitment Systems for Analysis of Protein-Protein Interactions.
Keywords: Cell biology; Cell Biology