|
|
|
|
| LEADER |
03438nam a22002895i 4500 |
| 001 |
978-1-59259-233-3 |
| 005 |
20191026231119.0 |
| 007 |
cr nn 008mamaa |
| 008 |
100301s2001 xxu| s |||| 0|eng d |
| 020 |
|
|
|a 9781592592333
|
| 024 |
7 |
|
|a 10.1385/1592592333
|2 doi
|
| 040 |
|
|
|a Sistema de Bibliotecas del Tecnológico de Costa Rica
|
| 245 |
1 |
0 |
|a Nuclease Methods and Protocols
|c edited by Catherine H. Schein.
|
| 250 |
|
|
|a 1st ed. 2001.
|
| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2001.
|
| 300 |
|
|
|a XVII, 525 p.
|b online resource.
|
| 336 |
|
|
|a text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a computer
|b c
|2 rdamedia
|
| 338 |
|
|
|a online resource
|b cr
|2 rdacarrier
|
| 490 |
1 |
|
|a Methods in Molecular Biology,
|v 160
|
| 505 |
0 |
|
|a Characterizing Nuclease Activity -- Processivity of DNA Repair Enzymes -- Analysis by HPLC of Distributive Activities and the Synthetic (Back) Reaction of Pancreatic-Type Ribonucleases -- Ier1 p: A Kinase and Site-Specific Endoribonuclease -- Microtiter-Plate Assay and Related Assays for Nonspecific Endonucleases -- Quantitating mRNAs with Relative and Competitive RT-PCR -- Expressing Self-Incompatibility RNases (S-RNases) in Transgenic Plants -- Molecular Cloning, Tissue Distribution, and Chromosomal Localization of the Human Homolog of the R2/Th/Stylar Ribonuclease Gene Family -- Inhibitors and Activators of Nucleases -- Ribonuclease Inhibitors -- Producing Soluble Recombinant RNases and Assays to Measure Their Interaction with Interferon-? In Vitro? -- Assays for the Evaluation of HIV-1 Integrase Inhibitors -- The Direction of Ribonucleases H by Antisense Oligodeoxynucleotides -- The 2-5A/RNase L Pathway and Inhibition by RNase L Inhibitor (RLI) -- Relating Nucleas Structure and Function -- Crystallization and Crystal Structure Determination of Ribonuclease A-Ribonuclease Inhibitor Protein Complex -- Methods for Studying the Interaction of Barnase with Its Inhibitor Barstar -- Assaying In Vitro Refolding of RNases by Mass Spectrometry -- Dissecting Nucleases into Their Structural and Functional Domains -- Using Electrostatics to Define the Active Site of Serratia Endonuclease -- Homology Modeling and Simulations of Nuclease Structures -- Methods for Determining Activity and Specificity of DNA Binding and DNA Cleavage by Class II Restriction Endonucleases -- Engineered Properties and Assays for Human DNase I Mutants -- Nucleases in the Clinic -- Assays for Human DNase I Activity in Biological Matrices -- Evaluation of Retroviral Ribonuclease H Activity -- Assays for Detection of RNase A Superfamily Ribonucleases -- Assay for Antitumor and Lectin Activity in RNase Homologs -- Isolation and Enzymatic Activity of Angiogenin -- Preparation and Preclinical Characterization of RNase-Based Immunofusion Proteins -- Assays Using Nucleases -- Restriction Endonucleases and Their Uses -- Isolation and Characterization of an Unknown Restriction Endonuclease -- Gene Modification with Hapaxoterministic Restriction Enzymes -- Techniques to Measure Nucleic Acid-Protein Binding and Specificity -- Analyzing the Developmental Expression of Sigma Factors with S1-Nuclease Mapping -- Detection and Quantitation of mRNAs Using Ribonuclease Protection Assays.
|
| 650 |
|
0 |
|a Cell biology.
|
| 650 |
1 |
4 |
|a Cell Biology.
|
| 700 |
1 |
|
|a Schein, Catherine H.
|e editor.
|
| 710 |
2 |
|
|a SpringerLink (Online service)
|
| 773 |
0 |
|
|t Springer eBooks
|
| 856 |
4 |
0 |
|u https://doi.org/10.1385/1592592333
|