Vascular Disease Molecular Biology and Gene Transfer Protocols - Baker, Andrew H.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1999)
Genetics in Vascular Disease -- Detection of Mutations and DNA Polymorphisms in Genes Involved in Cardiovascular Diseases by Polymerase Chain Reaction-Single-Strand Conformation Polymorphism Analysis -- Analysis of Genetic Variants in Cardiovascular Risk Genes by Heteroduplex Analysis -- Radiation Hybrid (RH) Mapping of Human Smooth Muscle-Restricted Genes -- Isolation of Genes Expressed in Vasular Tissue -- Efficient Extraction of RNA from Vascular Tissue -- Preparation of cDNA Libraries from Vascular Cells -- Screening cDNA Libraries Using Partial Probes to Isolate Full-Length cDNAs from Vascular Cells -- Cloning Full-Length cDNAs from Vascular Tissues and Cells by Rapid Amplification of cDNA Ends (RACE) and RT-PCR -- Use of cDNA Representational Difference Analysis to Identify Disease-Specific Genes in Human Atherosclerotic Plaques -- The Use of Differential mRNA Display (DDRT-PCR) to Identify Genes Differentially Expressed in Normal and Diseased Vascular Cells -- Identification of Novel Protein Kinases in Vascular Cells -- Methods for Mapping Transcriptional Start Sites and Measurement of Promoter Activity in Vascular Cells -- Primer Extension Analysis to Map Transcription Start Sites of Vascular Genes -- Use of Liposome-Mediated DNA Transfection to Determine Promoter Activity in Smooth Muscle Cells -- Nuclear Run-On Assay to Study Gene Transcription in Vascular Cells -- Electromobility Shift Analysis (EMSA) Applied to the Study of NF-kappa B Binding Interactions in Vascular Smooth Muscle Cells -- Molecular Analysis of MRNA Expression in Vascular Cells -- Measurement of Gene Expression in the Vascular Wall by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Analysis -- Northern Blot Analysis to Quantify Gene Expression in Vascular Diseases -- Localization of Gene Expression in Human Atherosclerotic Lesions by In SituHybridization -- RNase Protection Assays for Quantitation of Gene Expression in Vascular Tissue -- Molecular Methods to Study Apoptosis and Phenotypic Changes in Vascular Cells and Tissus -- Detection of Apoptosis in Atherosclerosis and Restenosis by Terminal dUTP Nick-End Labeling (TUNEL) -- In Vitro Detection of Apoptosis in Isolated Vascular Cells -- Molecular Assessment of the Phenotypic Changes Associated with Smooth Muscle Cells Using Two-Dimension Electrophoresis and Microsequence Analysis -- In Vitro Methods to Express Foreign Genes in Vasular Cells -- Simple Methods for Preparing Recombinant Adenoviruses for High-Efficiency Transduction of Vascular Cells -- Generation of Recombinant Adeno-Associated Viruses for Delivery of Genes into Vascular Cells -- Hemagglutinating Virus of Japan Liposome-Mediated Gene Delivery to Vascular Cells -- High-Efficiency and Low-Toxicity Adenovirus-Assisted Endothelial Transfection -- Gene Transfer in Vascular Cells Using an Engineered Na-H Exchanger (NHE1) as a Selectable Marker -- Use of Retroviruses to Express Exogenous Genes in Vascular Smooth Muscle Cells -- Embryonal Stem (ES) Cell-Derived Macrophages -- Delivery of Recombinant Adenoviruses to Human Saphenous Vein -- In Vivo Vascular Gene Transfer Protocols -- An In Vivo Angiogenesis Assay to Study Positive and Negative Regulators of Neovascularization -- Efficient Liposome-Mediated Gene Transfer to Rabbit Carotid Arteries In Vivo -- Gene Delivery to Rabbit Arteries Using the Collar Model -- Delivery of Antisense Oligonucleotides to the Vascular Wall -- Local Gene Delivery of Recombinant Adenoviruses to the Rat Carotid Artery In Vivo.
Keywords: Vascular surgery; Vascular Surgery