Hemostasis and Thrombosis Protocols - Perry, David J.; Pasi, K. John.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1999)
Hemostasis -- Basic Techniques -- Isolation of DNA and RNA -- Amplification of DNA and RNA by PCR -- Direct Sequencing of PCR Products -- Solid-Phase Sequencing of Biotinylated PCR Products with Streptavidin-Coated Magnetic Beads -- Automated DNA Sequencing -- Detection of DNA by Silver Staining -- Promoter Studies in Hemostasis -- Methods of Mutational Analysis -- Detection of Mutations and Polymorphisms in Clotting Factors by Denaturing Gradient Gel Electrophoresis -- Screening for Mutations in DNA by Single-Stranded Conformation Polymorphism (SSCP) Analysis -- Screening for DNA Heteroduplexes in the Factor VII Gene Using Ethylene Glycol Gel Electrophoresis of Solvent-Treated 32P-Labeled PCR Products -- Detection of Mutations Causing Hemophilia A Using an In Vitro Coupled Transcription and Translation System -- Screening for Mutations in the Human Antithrombin Gene by Hydrolink D-5000™ and MDE™ Gel Electrophoresis -- Methods for Analyzing Inherited/Acquired Disorders of Hemostasis -- Detection of Mutations in Hemophilia A Patients by Chemical Cleavage of Mismatch Method -- Inversion Mutation Analysis in Hemophilia A by Restriction Enzyme Analysis and Southern Blotting -- Hemophilia B Mutational Analysis -- Screening for Candidate Mutations Causing von Willebrand's Disease (vWD) -- Use of Intron 40 VNTR I in vWD Gene Tracking -- Multimeric Analysis of von Willebrand Factor -- Identification of Mutations in the Human Factor VII Gene -- Molecular Analysis in Factor XI Deficiency -- Mutational Analysis in Antithrombin Deficiency -- Ectopic Transcript Analysis in Human Antithrombin Deficiency -- Mutational Analysis of the Human Protein C Gene -- Analysis of the Protein S Gene in Protein S Deficiency -- Screening for the G to A Transition at Position 20210 in the 3?-Untranslated Region (UTR) of the Prothrombin Gene -- Screening for the Factor V Leiden Mutation -- Multiplex PCR for Detection of the Prothrombin 3?-UTR (G20210A) Polymorphism and the Factor V Leiden Mutation -- Isoelectric Focusing and Immunodetection of Plasma Antithrombin -- Characterization of Heparin Binding Variants of Antithrombin by Crossed Immunoelectrophoresis in the Presence of Heparin -- The Determination of Amino Acid Sequence Abnormalities in Proteins by HPLC Peptide Analysis -- Platelet and Megakaryocyte Analysis -- Molecular Biological Identification and Characterization of Inherited Platelet Receptor Disorders -- In Vitro Expansion of Megakaryocytes from Peripheral Blood Hematopoietic Progenitors -- Molecular Biology Studies with Primary Megakaryocytes.
Keywords: Pathology; Pathology