Calcium Signaling Protocols - Lambert, David G.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1999)
General -- Fluorescent Measurement of [Ca2+]c -- Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2 -- Measurement of [Ca2+]i in Cell Suspensions Using Indo-1 -- Specialist Measurement Systems -- Confocal Microscopy -- Measurement of Intracellular Calcium Concentration Using Confocal Microscopy -- Single Cell and Subcellular Measurement of Intracellular Ca2+ Concentration ([Ca2+]i) -- Measurement of [Ca2+] Using the Fluorometric Imaging Plate Reader (FLIPR) -- Nonelectrophysiological measurement of Ca2+ channel activity -- Measurement of Ca2+ Entry Using 45Ca2+ -- Measurement of [3H]PN200-110 and [125I]?-Conotoxin MVIIA Binding -- Measurement of Ins(1,4,5)P3 and Ca2+ Release from Intracellular Stores -- Measurement of Inositol (Poly)phosphate Formation Using [3H]Inositol Labeling Protocols in Permeabilized Cells -- Measurement of Inositol(1,4,5)trisphosphate Using a Stereospecific Radioreceptor Mass Assay -- Measurement of Calcium Fluxes in Permeabilized Cells Using a 45Ca2+ Uptake and Release Assay -- Microinjection of myo-Inositol(1,4,5)trisphosphate and Other Calcium-Mobilizing Agents into Intact Adherent Cells -- Photolysis of Caged Calcium Using a Low-Cost Flash Unit -- Measurement of Ca2+ Flux Through Ins(1,4,5)P3 Receptor-Ca2+ Channels in Lipid Bilayers ("Dip-Tip" and "Schindler" Methodology) -- Continuous Fluorescent Monitoring of Cellular Calcium Fluxe -- Measurement of Free [Ca2+] Changes in Agonist-Sensitive Internal Stores Using Compartmentalized Fluorescent Indicators -- Specialist Measurement Techniques -- Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry -- Measurement of Calcium and Movement in Heart Cells -- Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations -- Measurement of Cytosolic-Free Ca2+ in Plant Tissue -- Ca2+ Sensitive Targets -- Assay and Purification of Calmodulin-Dependent Protein Kinase -- Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1).
Keywords: Biochemistry; Biochemistry, general