Quantitative PCR Protocols - Kochanowski, Bernd; Reischl, Udo.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1999)
Reviews -- Quantitative PCR -- General Principles of Quantitative PCR -- Effects of Collection, Processing, and Storage on RNA Detection and Quantification -- Quantitative RT-PCR -- Kinetic Quantitative PCR vs End-Point Quantitative PCR with Internal Standard -- Comparison of Competitive PCR and Positive Control-Based PCR -- Protocols -- End-Point Titration-PCR for Quantitation of Cytomegalovirus DNA -- Analysis of Amplified DNA Molecules by Capillary Electrophoresis and Laser Induced Fluorescence -- Competitor Calibration and Analysis of Competitive Amplified PCR Products by High-Performance Liquid Chromatography (HPLC) -- Quantifying Amplicons with ELISA -- Competitive PCR Quantitation Utilizing a Microtiter Plate Based Format for the Detection of PCR Products -- Competitive and Differential RT-PCR (CD-RT-PCR) for Measurement of Normalized Gene Expression Using Antisense Competitors -- Amplified Assay for Specific Dual-Labeled DNA Using the Coagulation Cascade (EDNA-ELCA) -- Quantitative PCR with Internal Standardization and OLA-ELISA Product Analysis for the p53 Tumor Suppressor Gene -- Quantitative Analysis of Human DNA Sequences by PCR and Solid-Phase Minisequencing -- High Resolution PCR Quantitation by AmpliSensor Assay -- Construction of Polycompetitors for Competitive PCR -- Tailed RT-PCR for the Quantitation of Chloramphenicol Acetyl Transferase (CAT)mRNA -- A Stochastic PCR Approach for RNA Quantification in Multiple Samples -- Quantitation of mRNA Species by RT-PCR on Total mRNA Population with Nonradioactive Probes.
Keywords: Cell biology; Cell Biology