Eicosanoid Protocols - Lianos, Elias A.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1999)
Use of a Retroviral Vector for Expression of a Foreign Gene -- Extraction of Eicosanoids from Biological Fluids, Cells, and Tissues -- Assessment of Cyclooxygenase RNA Expression by Northern Hybridization -- Immunohistochemical Analysis of Cyclooxygenase Expression in Human Skin -- Separation of Hydroxyeicosatetraenoic Acids and Leukotrienes Originating from Kidney Tissue by C-18 Reverse-Phase High-Pressure Liquid Chromatography -- Localization and Distribution of Cyclooxygenase-2 in Brain Tissue by Immunohistochemistry -- Assessment of Cyclooxygenase Protein Expression by Western Blotting -- Assessment of Arachidonic Acid Distribution into Phospholipids of Inflammatory Cells -- Determination of Phospholipase A2s Expression in Mast Cells by Reverse-Transcriptase Polymerase Chain Reaction -- Enzyme immunoassays (EIA) of Eicosanoids -- Transcellular Regulation of Eicosanoid Biosynthesis -- Assessment of Cellular Localization of the Thromboxane A2 Receptor by Immunocytochemistry -- Epoxyeicosatrienoic Acids -- Assessment of the Expression of Prostaglandin Synthase-2 in Swiss 3T3 Fibroblasts -- Assessment of 5-Lipoxygenase Activity and Cellular Distribution -- Leukotrienes, Lipoxins, and Hydroxyeicosatetraenoic Acids -- Whole Blood Assays for Evaluation of Thromboxane Synthase Inhibition -- Novel Eicosanoids.
Keywords: Biochemistry; Biochemistry, general