Malaria Methods and Protocols - Doolan, Denise L.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2002)
Assessing Risk of Infection and Severity of Disease -- Vector Incrimination and Entomological Inoculation Rates -- Epidemiological Measures of Risk of Malaria -- Laboratory Models -- Maintenance of the Plasmodium berghei Life Cycle -- Mouse Models for Pre-Erythrocytic-Stage Malaria -- Mouse Models for Erythrocytic-Stage Malaria -- Nonhuman Primate Models -- Nonhuman Primate Models -- Diagnosis and Typing -- Vector Analysis -- Genotyping of Plasmodium spp. -- Genotyping of Plasmodium falciparum -- Microsatellite Analysis in Plasmodium falciparum -- Quantitation of Liver-Stage Parasites by Automated TaqMan® Real-Time PCR -- Quantitation of Liver-Stage Parasites by Competitive RT-PCR -- Molecular Biology Techniques -- Extraction and Purification of Plasmodium Total RNA -- Extraction and Purification of Plasmodium Parasite DNA -- Southern Blotting of Parasite DNA -- SDS-PAGE and Western Blotting of Plasmodium falciparum Proteins -- Nested PCR Analysis of Plasmodium Parasites -- RFLP Analysis -- Analysis of Gene Expression by RT-PCR -- In Situ Detection of RNA in Blood- and Mosquito-Stage Malaria Parasites -- Purification of Chromosomes from Plasmodium falciparum -- Construction of Genomic Libraries from the DNA of Plasmodium Species -- Construction of a Gene Library with Mung Bean Nuclease-Treated Genomic DNA -- Construction of Plasmodium falciparum ? cDNA Libraries -- Production of Stage-Specific Plasmodium falciparum cDNA Libraries Using Subtractive Hybridization -- Construction and Screening of YAC Libraries -- Episomal Transformation of Plasmodium berghei -- Gene Targeting in Plasmodium berghei -- Immunological Techniques -- Peptide Vaccination -- DNA Vaccination -- Assessing Antigen-Specific CD8+ and CD4+ T-Cell Responses in Mice After Immunization with Recombinant Viruses -- Assessing CD4+Helper T-Lymphocyte Responses by Lymphoproliferation -- Limiting Dilution Analysis of Antigen-Specific CD4+ T-Cell Responses in Mice -- Cell Trafficking -- Assessing Antigen-Specific Proliferation and Cytokine Responses Using Flow Cytometry -- Cytokine Analysis by Intracellular Staining -- Assessment of Antigen-Specific CTL- and CD8+-Dependent IFN-? Responses in Mice -- Assessing Antigen-Specific CD8+ CTL Responses in Humans -- Human Antibody Subclass ELISA -- Systemic Nitric Oxide Production in Human Malaria -- Systemic Nitric Oxide Production in Human Malaria -- Cell Biology Techniques -- In Vitro Culture of Plasmodium Parasites -- Automated Synchronization of Plasmodium falciparum Parasites by Culture in a Temperature-Cycling Incubator -- Hepatic Portal Branch Inoculation -- Hepatocyte Perfusion, Isolation, and Culture -- Inhibition of Sporozoite Invasion -- Inhibition of Liver-Stage Development Assay -- T-Cell Mediated Inhibition of Liver-Stage Development Assay -- Antibody-Dependent Cellular Inhibition Assay -- Erythrocytic Malaria Growth or Invasion Inhibition Assays with Emphasis on Suspension Culture GIA -- Analysis of CSA-Binding Parasites and Antiadhesion Antibodies -- Analysis of the Adhesive Properties of Plasmodium falciparum-Infected Red Blood Cells Under Conditions of Flow -- Preparation of Adhesive Targets for Flow-Based Cytoadhesion Assays -- Triton X-114 Phase Partitioning for Antigen Characterization -- Immunoprecipitation for Antigen Localization -- Testing Interventions For Malaria Control -- Field Trials.
Keywords: Infectious diseases; Infectious Diseases