Mitochondrial DNA Methods and Protocols - Copeland, William C.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2002)
Methods for the Analysis of MTDNA -- Animal Models for Mitochondrial Disease -- Identification of Mutations in mtDNA from Patients Suffering Mitochondrial Diseases -- Screening for Aging-Dependent Point Mutations in mtDNA -- Scanning Low-Frequency Point Mutants in the Mitochondrial Genome Using Constant Denaturant Capillary Electrophoresis -- Identification of mtDNA Mutations in Human Cancer -- In Situ PCR of the Common Human mtDNA Deletion -- Reproducible Quantitative PCR of Mitochondrial and Nuclear DNA Copy Number Using the LightCycler™ -- Yeast Nuclear Genes for mtDNA Maintenance -- Measuring mtDNA Mutation Rates in Saccharomyces cerevisiae Using the mtArg8 Assay -- Measuring Oxidative mtDNA Damage and Repair Using Quantitative PCR -- Migration of mtDNA into the Nucleus -- 2D Gel Electrophoresis of mtDNA -- Methods for the Analysis of proteins involved in MTDNA Transactions -- Purification of Mitochondria for Enzymes Involved in Nucleic Acid Transactions -- Purification of Mitochondrial Uracil-DNA Glycosylase Using Ugi-Sepharose Affinity Chromatography -- Characterization of Specialized mtDNA Glycosylases -- Purification, Separation, and Identification of the Human mtDNA Polymerase With and Without Its Accessory Subunit -- Assay of mtDNA Polymerase ? from Human Tissues -- Purification Strategies for Drosophila mtDNA Replication Proteins in Native and Recombinant Form -- Purification Strategies for Drosophila mtDNA Replication Proteins in Native and Recombinant Form -- Expression, Purification, and In Vitro Assays of Mitochondrial Single-Stranded DNA-Binding Protein -- Recombinant Yeast mtDNA Helicases Purification and Functional Assays -- Isolation of DNA Topoisomerases I and II Activities from Mitochondria of Mammalian Heart -- 23 Endonuclease G Isolation and Assays -- 24 Targeting DNA Repair Proteins to Mitochondria -- 25 In Organello Footprinting of mtDNA -- Crosslinking of Proteins to mtDNA -- Breeding and Genotyping of Tfam Conditional Knockout Mice.
Keywords: Cell biology; Cell Biology