Protein Sequencing Protocols - Smith, Bryan John.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2003)
Strategies for Handling Polypeptides on a Micro-Scale -- SDS Polyacrylamide Gel Electrophoresis for N-Terminal Protein Sequencing -- Two-Dimensional Polyacrylamide Gel Electrophoresis for the Separation of Proteins for Chemical Characterization -- Electroblotting of Proteins from Polyacrylamide Gels for Chemical Characterization -- Enzymatic Cleavage of Proteins -- Chemical Cleavage of Polypeptides -- Enzymatic Preparation and Isolation of Glycopeptides -- Analytical and Micropreparative Capillary Electrophoresis of Peptides -- High-Performance Liquid Chromatography On-Line Derivative Spectroscopy for the Characterization of Peptides with Aromatic Amino Acid Residues -- Hydrolysis of Samples for Amino Acid Analysis -- Amino Acid Analysis -- Post Column Amino Acid Analysis -- Amino Acid Analysis Using Pre-Column Derivatization with 6-AminoquinolylN-Hydroxysuccinimidyl Carbamate -- Amino Acid Analysis in Protein Hydrolysates Using Anion Exchange Chromatography and IPAD Detection -- D-Amino Acid Analysis -- Validation of Amino Acid Analysis Methods -- Electrospray Mass Spectrometry of Peptides, Proteins, and Glycoproteins -- Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry -- Nanoelectrospray Tandem Mass Spectrometry and Sequence Similarity Searching for Identification of Proteins from Organisms with Unknown Genomes -- Direct Identification of Proteins in Ultracomplex Mixtures -- Identification of PTH-Amino Acids by HPLC -- Protein Sequencer Maintenance and Troubleshooting -- N-Terminal Protein Sequencing for Special Applications -- Identification of Phosphorylation Sites by Edman Degradation -- Validation of Protein Sequencing in a Regulated Laboratory -- Automated C-Terminal Protein Sequence Analysis Using the Alkylated-Thiohydantoin Method -- Chemical Modifications of Proteins as an Aid to Sequence Analysis -- Deblocking of N-Terminally Modified Proteins -- Deblocking of Proteins Containing N-Terminal PyroglutamicAcid -- Identification of Sites of Glycosylation -- Analysis of Sites of Protein Phosphorylation -- Quantitation and Location of Disulfide Bonds in Proteins -- Getting the Most from Your Protein Sequence -- Letter Codes, Structures, Masses, and Derivatives of Amino Acids -- Protein Consensus Sequence Motifs.
Keywords: Biochemistry; Biochemistry, general