PCR Protocols - Bartlett, John M. S.; Stirling, David; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2003)
to PCR -- A Short History of the Polymerase Chain Reaction -- PCR Patent Issues -- Equipping and Establishing a PCR Laboratory -- Quality Control in PCR -- Preparation of Nucleic Acid Templates -- Extraction of Nucleic Acid Templates -- Extraction of DNA from Whole Blood -- DNA Extraction from Tissue -- Extraction of DNA from Microdissected Archival Tissues -- RNA Extraction from Blood -- RNA Extraction from Frozen Tissue -- RNA Extraction from Tissue Sections -- Dual DNA/RNA Extraction -- DNA Extraction from Fungi, Yeast, and Bacteria -- Isolation of RNA Viruses from Biological Materials -- Extraction of Ancient DNA -- DNA Extraction from Plasma and Serum -- Technical Notes for the Detection of Nucleic Acids -- Technical Notes for the Recovery and Purification of PCR Products from Acrylamide Gels -- Basic PCR Methods -- PCR Primer Design -- Optimization of Polymerase Chain Reactions -- Subcycling PCR for Long-Distance Amplifications of Regions with High and Low Guanine-Cystine Content -- Rapid Amplification of cDNA Ends -- Randomly Amplified Polymorphic DNA Fingerprinting -- Microsphere-Based Single Nucleotide Polymorphism Genotyping -- Ligase Chain Reaction -- Nested RT-PCR in a Single Closed Tube -- Direct PCR from Serum -- Long PCR Amplification of Large Fragments of Viral Genomes -- Long PCR Methodology -- Ultrasensitive and Quantitative PCR -- Qualitative and Quantitative PCR -- Ultrasensitive PCR Detection of Tumor Cells in Myeloma -- Ultrasensitive Quantitative PCR to Detect RNA Viruses -- Quantitative PCR for cAMP RI Alpha mRNA -- Quantitation of Multiple RNA Species -- Transcriptome Analysis -- Differential Display -- AU-Differential Display, Reproducibility of a Differential mRNA Display Targeted to AU Motifs -- PCR Fluorescence Differential Display -- Microarray Analysis Using RNA Arbitrarily Primed PCR -- Oligonucleotide Arrays for Genotyping -- Serial Analysis of Gene Expression -- Mutations and Polymorphisms -- Mutation and Polymorphism Detection -- Combining Multiplex and Touchdown PCR for Microsatellite Analysis -- Detection of Microsatellite Instability and Loss of Heterozygosity Using DNA Extracted from Formalin-Fixed Paraffin-Embedded Tumor Material by Fluorescence-Based Multiplex Microsatellite PCR -- Reduction of Shadow Band Synthesis During PCR Amplification of Repetitive Sequences from Modern and Ancient DNA -- Degenerate Oligonucleotide-Primed PCR -- Mutation Detection Using RT-PCR-RFLP -- Multiplex Amplification Refractory Mutation System for the Detection of Prothrombotic Polymorphisms -- PCR-SSCP Analysis of Polymorphism -- PCR-Based Sequencing -- Sequencing -- Preparation and Direct Automated Cycle Sequencing of PCR Products -- Nonradioactive PCR Sequencing Using Digoxigenin -- Direct Sequencing by Thermal Asymmetric PCR -- Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing -- Direct Sequencing with Highly Degenerate and Inosine-Containing Primers -- Determination of Unknown Genomic Sequences Without Cloning -- Cloning PCR Products for Sequencing in M13 Vectors -- DNA Rescue by the Vectorette Method -- Technical Notes for Sequencing Difficult Templates -- In Situ PCR and Prins -- PCR-Based Detection of Nucleic Acids in Chromosomes, Cells, and Tissues -- Cycling Primed In Situ Amplification -- Direct and Indirect In Situ PCR -- Reverse Transcriptase In Situ PCR -- Primed In Situ Nucleic Acid Labeling Combined with Immunocytochemistry to Simultaneously Localize DNA and Proteins in Cells and Chromosomes -- Cloning and Mutagenesis -- Cloning and Mutagenesis -- Using T4 DNA Polymerase to Generate Clonable PCR Products -- A T-Linker Strategy for Modification and Directional Cloning of PCR Products -- Cloning Gene Family Members Using PCR with Degenerate Oligonucleotide Primers -- cDNA Libraries from a Low Amount of Cells -- Creation of Chimeric Junctions, Deletions, and Insertions by PCR -- Recombination and Site-Directed Mutagenesis Using Recombination PCR -- Megaprimer PCR.
Keywords: Cell biology; Cell Biology