Protein Misfolding and Disease - Bross, Peter; Gregersen, Niels; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2003)
General Concepts and Models -- Protein Misfolding, Aggregation, and Degradation in Disease -- Basic Introduction to In Vivo Protein Folding and Its Defects -- Cystic Fibrosis -- ?1-Antitrypsin Deficiency -- Parkinson's Disease -- Aberrant Protein Folding as the Molecular Basis of Cancer -- Yeast as a Model System to Investigate Protein Conformational Diseases -- General Methods -- Expression of Recombinant Proteins -- Expression in E. coli Systems -- Protein Expression in Yeasts -- Site-Directed Mutagenesis -- Pulse-Chase Labeling Techniques for the Analysis of Protein Maturation and Degradation -- Techniques in Conformational Disease Research -- Detection of Aggregates and Protein Inclusions by Staining of Tissues -- Study of Mutant Proteins With Folding Defects in Cultured Patient Cells -- E. coli Expression System for Identifying Folding Mutations of Human Adenosine Deaminase -- Characterization of Overexpressed Mutant Proteins in Mammalian Cells -- Investigation of Unfolded-Protein Response in Cells Expressing Familial Alzheimer's Disease-Linked Presenilin Variants -- Huntingtin Fragments Form Aggresome-Like Inclusion Bodies in Mammalian Cells -- Application of Chemical Chaperones to the Rescue of Folding Defects -- Analysis of Defective Subunit Interactions Using the Two-Hybrid System -- Investigation of Folding and Degradation of In Vitro Synthesized Mutant Proteins in the Cytosol -- Investigation of Folding and Degradation of In Vitro Synthesized Mutant Proteins in Microsomes -- Investigation of Folding and Degradation of In Vitro Synthesized Mutant Proteins in Mitochondria -- Investigation of Folding and Degradation of Mutant Proteins Synthesized in Semipermeabilized Cells.
Keywords: Biochemistry; Biochemistry, general