Membrane Protein Protocols Expression, Purification, and Characterization - Selinsky, Barry S.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2003)
Expression of Membrane Proteins -- Expression and Purification of the Amphipathic Form of Rabbit Cytochrome b5 in Escherichia coli -- Dihydroorotate Dehydrogenase of Escherichia coli -- A General Approach for Heterologous Membrane Protein Expression in Escherichia coli -- Expression of Membrane-Bound Iron-Sulfur Proteins -- Heterologous Expression of Human Scavenger Receptor Class B Type I (SR-BI) in Pichia pastoris -- Expression of Oligomeric Amiloride-Sensitive Epithelial Sodium Channel in Sf9 Insect Cells -- Functional Expression of His-Tagged Rhodopsin in Sf9 Insect Cells -- Detergent Selection in Membrane Protein Purification -- Preparation of Glycerol Facilitator for Protein Structure and Folding Studies in Solution -- Solubilization of Chemokine Receptors from Cell Membranes -- A Systematic Approach for the Solubilization of the Integral Membrane Protein Lysophospholipid -- Membrane Protein Purification -- Purification of Omp50, a Minor Porin of Campylobacter jejuni -- Purification of Porins from Mycobacterium smegmatis -- Isolation of the Melanocortin 5 Receptor -- Purification of Mammalian Serine Palmitoyltransferase, a Hetero-Subunit Enzyme for Sphingolipid Biosynthesis, by Affinity-Peptide Chromatography -- Purification of Phosphatidylglycerophosphate Synthase from Cultured Mammalian Cells -- Purification of Pancreastatin Receptor from Rat Liver Membranes -- Purification, Reconstitution, and Functional Characterization of Zinc Transporter from Rat Renal Brush Border Membranes -- Isolation of Lipid Raft-Associated Proteolipids -- Purification of Membrane-Bound Catechol-O-Methyltransferase -- Purification and Characterization of Transporter Proteins from Human Erythrocyte Membrane -- Purification of the Human Erythrocyte PS-Stimulated Mg2+-ATPase -- Reconstitution and Assay of Biogenic Membrane-Derived Phospholipid Flippase Activity in Proteoliposomes -- Expression, Purification, and Reconstitution of Rat Liver Carnitine Palmitoyltransferase I -- Structural Analysis of Membrane Proteins -- Crystallization in Lipidic Cubic Phases -- Optical Biosensor Assay Using Retroviral Receptor Pseudotypes.
Keywords: Biochemistry; Biochemistry, general