Nucleic Acids - Walker, John M.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1984)
The Burton Assay for DNA -- DABA Fluorescence Assay for Submicrogram Amounts of DNA -- Preparation of "RNase-Free" DNase by Alkylation -- The Isolation of Satellite DNA by Density Gradient Centrifugation -- The Isolation of High Molecular Weight Eukaryotic DNA -- Preparation of Lyophilized Cells to Preserve Enzyme Activities and High Molecular Weight Nucleic Acids -- Agarose Gel Electrophoresis of DNA -- Autoradiography of Gels Containing 32P -- Detection of Specific DNA Sequences-The Southern Transfer -- The Extraction and Isolation of DNA from Gels -- One-Dimensional Electrophoresis of Nucleic Acids in Agarose Using Denaturation with Formaldehyde and Identification of 3H-Labeled RNA by Fluorography -- Gel Electrophoresis of RNA in Agarose and Polyacrylamide Under Nondenaturing Conditions -- The Extraction of Total RNA by the Detergent and Phenol Method -- RNA Extraction by the Proteinase K Method -- RNA Extraction by the Guanidine Thiocyanate Procedure -- The Purification of Poly(A)-Containing RNA by Affinity Chromatography -- Messenger RNA Fractionation on Neutral Sucrose Gradients -- The Estimation of mRNA Content by Poly(U) Hybridization -- DNA Directed in Vitro Protein Synthesis with Escherichia coli S-30 Extracts -- In Vitro Translation of Messenger RNA in a Wheat Germ Extract Cell-Free System -- In Vitro Translation of Messenger RNA in a Rabbit Reticulocyte Lysate Cell-Free System -- Immunoprecipitation of in Vitro Translation Products with Protein A Bound to Sepharose -- In Vitro Continuation of RNA Synthesis Initiated in Vivo -- Synthesis of Double-Stranded Complementary DNA from Poly(A)+mRNA -- Plasmid DNA Isolation by the Cleared Lysate Method -- Plasmid DNA Isolation (Sheared Lysate Method) -- Small-Scale Plasmid DNA Preparation -- Preparation of Chromosomal DNA from E. coli -- Preparation and Assay of Phage Lambda -- Preparation of Phage Lambda DNA -- The Use of Restriction Endonucleases -- Ligation of DNA with T4 DNA Ligase -- The Use of Alkaline Phosphatase to Prevent Vector Regeneration -- Bacterial Transformation -- Bacterial Transformation (Kushner Method) -- In Vitro Packaging of DNA -- Yeast Transformation -- Radiolabeling of DNA by Nick Translation -- Radiolabeling of DNA Using Polynucleotide Kinase -- Radiolabeling of DNA with 3? Terminal Transferase -- Radiolabeling of DNA with the Klenow Fragment of DNA Polymerase -- Identification of Recombinant Plasmids by In Situ Colony Hybridization -- Identification of Recombinant Phages by Plaque Hybridization -- Plasmid Screening Using Single Colony Lysates -- Immunological Detection of Gene Expression in Recombinant Clones -- The Isolation of Minicells -- The Isolation of Maxicells -- The Identification of Gene Products in Minicells and Maxicells -- Molecular Cloning in Bacteriophage Lambda and in Cosmids -- DNA Transformation of Mammalian Cells -- Chemical Cleavage (Maxam and Gilbert) Method for DNA Sequence Determination -- Gel Electrophoretic Analysis of DNA Sequencing Products -- DNA Sequence Determination Using Dideoxy Analogs.
Keywords: Biochemistry; Biochemistry, general