Gene Transfer and Expression Protocols - Murray, Edward J.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1991)
Preparation of Recombinant Plasmid DNA for DNA-Mediated Gene Transfer -- Calcium Phosphate Mediated Gene Transfer into Established Cell Lines -- Transfection of the ChloramphenicolAcetyltransferase Gene into Eukaryotic Cells Using Diethyl-Aminoethyl (DEAE)-Dextran -- Polybrene/DMSOAssisted Gene Transfer -- Electroporation Technique of DNA Transfection -- Irradiation and Fusion Gene Transfer -- Direct Use of ? Phage Particles for DNA Transfection -- Cationic Liposome-Mediated Transfection with Lipofectin™ Reagent -- Preparation of High-Molecular-Weight DNA for Use in DNA Transfection -- Chromosome-Mediated Gene Thnsfer -- Manipulation of Adenovirus Vectors -- Manipulation of Vaccinia Virus Vectors -- Manipulation of Baculovirus Vectors -- Manipulation of SV40 Vectors -- Choice and Manipulation of Retroviral Vectors -- Use of Tissue-Plasminogen Activator as a Reporter Gene -- Use of Escherichiu coli (E. coli) lacZ (?-Galactosidase) as a Reporter Gene -- Application of the Firefly Luciferase Reporter Gene -- Use of Vectors to Confer Resistance to Antibiotics G418 and Hygromycin in Stably Transfected Cell Lines -- Selection of Cells Defective in Pyrimidine (TK?) and Purine (APRT? and HPRT? Salvage -- Quantitative and Qualitative Analysis of Exogenous Gene Expression by the S1 Nuclease ProtectionAssay -- The RNase Protection Assay -- Primer Extension Analysis of mRNA Isolated from Transfected Cell Lines -- Northern Blot Analysis of Gene Expression -- Analysis of Transcriptional Initiation in Isolated Nuclei -- Immunoperoxidase Staining of Gene Products in Cultured Cells Using Monoclonal Antibodies -- The Use of Flow Cytometry to Detect Transfected Gene Products -- Determination of Foreign Gene Copy Number in Stably Transfected Cell Lines by Southern Transfer Analysis -- Evaluation of Extrachromosomal Gene Copy Number of Transiently Transfected Cell Lines -- Use of Dpn I Restriction Enzyme to Assess Newly Replicated Gene Copies in Amplifiable Vector Systems -- Use of Polymerase Chain Reaction (PCR) to Detect Homologous Recombination in Transfected Cell Lines -- Induction of Erythroid-Specific Expression in Murine Erythroleukemia (MEL) Cell Lines.
Keywords: Human genetics; Human Genetics