PCR Protocols Current Methods and Applications - White, Bruce A.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1993)
Polymerase Chain Reaction -- Selection of Primers for Polymerase Chain Reaction -- Direct Radioactive Labeling of Polymerase Chain Reaction Products -- Use of Arithmetic Polymerase Chain Reaction for Synthesis of Single-Stranded Probes for S1 Nuclease Assays -- Nonradioactive Labeling of Polymerase Chain Reaction Products -- Quantitation and Purification of Polymerase Chain Reaction Products by High-Performance Liquid Chromatography -- Use of Polymerase Chain Reaction for Screening Transgenic Mice -- Polymerase Chain Reaction Analysis of DNA from Paraffin-Embedded Tissue -- The Use of Polymerase Chain Reaction for Chromosome Assignment -- Mapping MHC Class II Genes and Disease-Susceptibility -- The Use of the Polymerase Chain Reaction and the Detection of Amplified Products -- Determination of Loss of Heterozygosity Using Polymerase Chain Reaction -- Direct Sequencing of Polymerase Chain Reaction Products -- Manual and Automated Direct Sequencing of Product Generated by the Polymerase Chain Reaction -- Genomic Footprinting by Ligation Mediated Polymerase Chain Reaction -- RNA Template-Specific Polymerase Chain Reaction (RS-PCR) -- Quantitative Measurement of Relative Gene Expression in Human Tumors -- Identification of Alternatively Spliced mRNAs and Localization of 5' Ends by Polymerase Chain Reaction Amplification -- Utilization of Polymerase Chain Reaction for Clonal Analysis of Gene Expression -- Sequencing DNA Amplified Directly from a Bacterial Colony -- Use of Polymerase Chain Reaction to Screen Phage Libraries -- Molecular Cloning of Polymerase Chain Reaction Fragments with Cohesive Ends -- Rapid (Ligase-Free) Subcloning of Polymerase Chain Reaction Products -- Use of Polymerase Chain Reaction for Making Recombinant Constructs -- In Vitro Recombination and Mutagenesis of DNA -- Use of Polymerase Chain Reaction for the Rapid Construction of Synthetic Genes -- Recombinant Circle Polymerase Chain Reaction for Site-Directed Mutagenesis -- Site-Directed Mutagenesis by Double Polymerase Chain Reaction -- Generation of a Polymerase Chain Reaction Renewable Source of Subtractive cDNA -- PCR-Based Full-Length cDNA Cloning Utilizing the Universal-Adaptor/Specific DOS Primer-Pair Strategy -- Use of Degenerate Oligonucleotide Primers and the Polymerase Chain Reaction to Clone Gene Family Members -- Single Specific Primer-Polymerase Chain Reaction (SSP-PCR) and Genome Walking -- cDNA Cloning by Inverse Polymerase Chain Reaction -- Amplification of Gene Ends from Gene Libraries by Polymerase Chain Reaction with Single-Sided Specificity -- Anchoring a Defined Sequence to the 5? Ends of mRNAs.
Keywords: Cell biology; Cell Biology