Protocols in Molecular Parasitology - Hyde, John E.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1993)
Culturing and Biological Cloning of Trypanosoma brucei -- Culturing and Biological Cloning of Trypanosoma cruzi -- In Vitro Cultivation and Biological Cloning of Leishmania -- Simple In Vitro Cultivation of the Malaria Parasite Plasmodium falciparum (Erythrocytic Stages) -- Synchronization and Cloning of Malaria Parasites -- The Culture and Preparation of Gametocytes of Plasmodium falciparum for Immunochemical, Molecular, and Mosquito Infectivity Studies -- The Culture of Schistosoma mansoni and Production of Life Cycle Stages -- Preparation of DNA and RNA from Trypanosoma brucei -- Isolation of RNA and DNA from Trypanosoma cruzi -- Isolation of DNA and RNA from Leishmania -- The Extraction and Purification of DNA and RNA from In Vitro Cultures of the Malaria Parasite Plasmodium falciparum -- Extraction and Purification of Nucleic Acids from Schistosomes -- Application of DNA and Whole Organisms to Filter Supports for DNA Probe Analysis: Dot, Slot, and Touch Blotting -- The Development and Use of Repetitive Sequences as DNA Probes for Parasite Detection and Species Identification -- Analysis of the DNA of Parasitic Protozoa by Flow Cytometry -- PCR orLowLevel Detection of Malaria Parasites in Blood -- PCR Methods for Identification of Point Mutations and Gene Rearrangements -- Antigenic Typing of Field Isolates of Plasmodium by DNA Techniques -- Ribosomal RNA Probes for Detection and Identification of Species -- Rapid Sequencing of Parasite rRNA -- Antibody Screening of Expression Libraries -- Antibody Select Procedure for Characterization of Expression Clones -- Isolation of Parasite Genes Using Synthetic Oligonucleotides -- Chromosome Mapping Methods for Parasitic Protozoa -- Transfection ofLeishmania and Trypanosoma brucei by Electroporation -- Two-Dimensional Polyacrylamide Gel Electrophoresis -- Isoenzyme Electrophoresis for Parasite Characterization -- The Surface Labeling of Schistosomes -- Monoclonal Antibody Affinity Chromatography -- Immunoblotting and Enzyme-Linked Immunosorbent Assay -- Immunofluorescence of Parasites -- Immunoelectron Microscopic Localization of Antigens in Malaria Parasites -- Preparation of Blotted Membrane for Protein Microsequencing -- Identification of T-Cell Epitopes.
Keywords: Microbiology; Microbiology