Protocols for Nucleic Acid Analysis by Nonradioactive Probes - Isaac, Peter G.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1994)
About Nonradioactive Nucleic Acid Detection -- Isolation of DNA from Plants -- Isolation of High-Molecular-Weight DNA from Animal Cells -- Restriction Enzyme Digestion, Gel Electrophoresis, and Vacuum Blotting of DNA to Nylon Membranes -- Isolation of Plant RNA -- Isolation of Total and Poly A+ RNA from Animal Cells -- Preparation of RNA Gel Blots -- Preparation of RNA Dot Blots -- Isolation of Plasmids for the Preparation of Probes -- Production of Hybridization Probes by the PCR Utilizing Digoxigenin-Modified Nucleotides -- Production of DNA Hybridization Probes with Digoxigenin-Modified Nucleotides by Random Hexanucleotide Priming -- Digoxigenin Labeling of RNA Transcripts from Multi- and Single-Locus DNA Minisatellite Probes -- Labeling of Double-Stranded DNA Probes with Biotin -- Preparation of Horseradish Peroxidase-Labeled Probes -- Random Prime Labeling of DNA Probes with Fluorescein-11-dUTP -- Labeling of Oligonucleotides with Fluorescein -- Hybridization of Digoxigenin-Labeled Probes to Southern Blots and Detection by Chemiluminescence -- Southern Blot Hybridization of Digoxigenin-Labeled RNA Minisatellite Probes and Color Detection -- Hybridization and Detection of Digoxigenin Probes on RNA Blots -- Hybridization of Horseradish Peroxidase-Labeled Probes and Detection by Enhanced Chemiluminescence -- Hybridization and Detection of Fluorescein-Labeled DNA Probes Using Enhanced Chemiluminescence -- Hybridization of Fluorescein-Labeled Oligonucleotide Probes and Enhanced Chemiluminescence Detection -- Preparation of Chromosome Spreads by Root-Tip Meristem Dissection for In Situ Hybridization with Biotin-Labeled Probes -- Enzymatic Treatment of Plant Material to Spread Chromosomes for In Situ Hybridization -- Use of Biotin-Labeled Probes on Plant Chromosomes -- Direct Fluorochrome-Labeled DNA Probes for Direct Fluorescent In Situ Hybridization to Chromosomes -- Detection of Digoxigenin-Labeled DNA Probes Hybridized to Plant Chromosomes In Situ -- Preparation of Tissue Sections and Slides for mRNA Hybridization -- Detecting mRNAin Tissue Sections with Digoxigenin-Labeled Probes -- Detection of mRNA in Whole Mounts of Mouse Embryos Using Digoxigenin Riboprobes -- PACE (Probe Assay-Chemiluminescence Enhanced) -- Detection of Foodborne Pathogens Using DNA Probes and a Dipstick Format -- Analysis of Gene Sequences by Hybridization of PCR-Amplified DNA to Covalently Bound Oligonucleotide Probes -- RAPD Assay -- Nonradioactive Oligonucleotide Probes for Detecting Products of the Ligase Chain Reaction -- Nucleic Acid Sequence-Based Amplification (NASBA™).
Keywords: Cell biology; Cell Biology