DNA'Protein Interactions Principles and Protocols - Kneale, G. Geoff; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1994)
DNase I Footprinting -- Footprinting with Exonuclease III -- Hydroxyl Radical Footprinting -- Hydroxyl Radical Interference -- 1,10-Phenanthroline-Copper Ion Nuclease Footprinting of DNA-Protein Complexes in Situ Following Mobility-Shift Electrophoresis Assays -- Identification of Protein-DNA Contacts with Dimethyl Sulfate -- Diethyl Pyrocarbonate as a Probe of Protein-DNA Interactions -- Osmium Tetroxide Modification and the Study of DNA-Protein Interactions -- Diffusible Singlet Oxygen as a Probe of DNA Deformation -- Ethylation Interference -- Uranyl Photofootprinting of DNA-Protein Complexes -- Nitration of Tyrosine Residues in Protein-Nucleic Acid Complexes -- Limited Proteolysis of Protein-Nucleic Acid Complexes -- Cloning and Expression of DNA Binding Domains Using PCR -- Overexpression and Purification of Eukaryotic Transcription Factors as Glutathione-S-Transferase Fusions in E. coli -- Site-Directed Mutagenesis by the Cassette Method -- Site-Directed and Site-Saturation Mutagenesis Using Oligonucleotide Primers -- UV Laser-Induced Protein-DNA Crosslinking -- Ultraviolet Crosslinking of DNA-Protein Complexes via 8-Azidoadenine -- Filter-Binding Assays -- The Gel Shift Assay for the Analysis of DNA-Protein Interactions -- Improved Plasmid Vectors for the Analysis of Protein-Induced DNA Bending -- Determination of Sequence Preferences of DNA Binding Proteins Using Pooled Solid-Phase Sequencing of Low Degeneracy Oligonucleotide Mixtures -- Analysis of DNA-Protein Interactions by Intrinsic Fluorescence -- A Competition Assay for DNA Binding Using the Fluorescent Probe ANS -- Circular Dichroism for the Analysis of Protein-DNA Interactions -- Electron Microscopy of Protein-Nucleic Acid Complexes -- Reconstitution of Protein-DNA Complexes for Crystallization -- Assay of Restriction Endonucleases Using Oligonucleotides -- Assays for Restriction Endonucleases Using Plasmid Substrates -- Assays for Transcription Factor Activity -- An Assay for In Vitro Recombination Between Duplex DNA Molecules.
Keywords: Biochemistry; Biochemistry, general